中文摘要：

催化元件以及由多个催化元件组成的合成途径的设计与组装为人工合成体系的建立奠定了基础，是合成生物学的重要研究内容。除从自然生物中挖掘大量的天然酶和途径可供人工合成体系使用外，将计算生物学、蛋白质工程以及组合生物合成等技术相结合，理性地、有目的地进行催化元件和途径的人工设计与组装，将提供新功能酶以及新物质合成途径。介绍了催化元件和合成途径人工设计与组装的研究策略和最新进展。

英文摘要：

Design of enzymes, as well as assembly of synthetic pathways, which are composed of multiple enzymes, are the basis for constructing artificial synthetic system, therefore they are important issues in synthetic biology. The researchers are demanded not only to explore the reservoirs of the natural enzymes but also design the new functional biocatalysts and assemble the novel synthetic pathways by combining the multidisciplinary theories and techniques, such as computational biology, protein engineering, combinatorial biosynthesis and so on. The research strategies and recent progress in artificial design and assembly of enzymes and synthetic pathways are reviewed here.