中文摘要：

将葡萄糖氧化酶（GOD）固定在多壁碳纳米管（MWCNTs）修饰电极（ME）上，GOD催化氧化葡萄糖生成过氧化氢，并使鲁米诺产生电致化学发光（ECL），据此构建了一种新型ECL葡萄糖传感器。结果表明：CNTs修饰的电极对鲁米诺和H2O2反应具有显著的电催化活性和增敏效果。该传感器对葡萄糖检测的线性范围为0．01—10．0mmol／L，相关系数R=0．999（n=5），检测限为5．0μmol／L。此传感器响应快，稳定性高，测定条件接近人体自然pH。将所建立的方法用于临床血清样品中葡萄糖含量的测定，获得了满意的结果。

英文摘要：

Incorporation of muhi-walled carbon nanotubes on the electrode is performed and modification of glassy carbon electrode with the prepared nano-hybrid material led to the fabrication of a novel electrode. The modified electrode shows attractive electrocatalytic activity and sensitizing effect on Luminol-H2O2 electrochemiluminescence （ECL） reactions at neutral media. The sensitized Luminol-H2O2 reactions are successfully applied for the ECL determination of glucose. Under the optimal conditions for Luminol-H2O2 system, the ECL signal intensity of luminol is linear with the concentration of glucose in the range of 0.01 -10.0 mmol/L,correlation coefficient R = 0. 999 （ n =5 ）, the limits of detection for glucose are 5.0μmol/L. Excellent electrocatalytic activity, high stability, technically simple and possibility of preparation at short period of time are of great advantages of this glucose biosensor.