【目的】探讨利用近红外光谱分析技术区分不同地域来源和饲养期牛肉的可行性,为牛肉产地溯源和产地确证等监管措施提供分析方法和理论依据。【方法】利用近红外光谱仪测定在内蒙古太仆寺旗、陕西杨凌区、河南南阳市实施的人为迁徙饲养模型试验中的18头肉牛肌肉样品的光谱,结合饲料和饲养方式,比较地域和饲养期样品之间的光谱特征差异,并利用主成分分析比较样品的空间分布。【结果】牛肉样品近红外光谱特征与地域、饲料种类和饲养方式等因子密切相关;同一地域不同饲养期和同一饲养期不同地域牛肉的近红外光谱特征均有显著差异;利用主成分分析能将不同地域来源和饲养期样品区分开。【结论】地域和饲养期对牛肉近红外光谱均有显著影响,进行牛肉产地溯源和产地确证时必须考虑地域和饲养期等因子;近红外光谱分析技术用于区分不同地域来源和饲养期的牛肉是可行的。
【Objective】The feasibility of using near infrared spectroscopy(NIRS) to differentiate beef from different regions and feeding periods was discussed,and the objective was to provide analytical methods and theoretical support for geographical origin assignment and authentication of beef.【Method】 Eighteen cattle muscle samples obtained from designed migration and feeding model experiment in Taipusiqi(Inner Mongolia Autonomous Region),Yangling Zone(Shaanxi province) and Nanyang City(Henan province) were scanned by DA7200 NIR spectrometer,and the differences of NIR spectra were analyzed.Principle component analysis was used to analyze the spatial distribution of samples.【Result】 NIR spectra of beef samples were closely related to factors such as region,feed and feeding practice,and there were significant differences in NIR spectra of samples from different regions and feeding periods.The results indicated that samples could be differentiated according to their regions and feeding periods.【Conclusion】NIR spectra of beef are significantly affected by regions and feeding periods,which have to be taken into account for geographical origin assignment and authentication.It is feasible to differentiate beef from different regions and feeding periods using NIRS analysis.