中文摘要：

应用PARASS（poly-AanchoredRNAaccessiblesitesscreening）技术筛选Fas基因mRNA获得3个潜在反义作用靶点，靶点1、2、3分别位于Fas基因297nt～317nt、619nt～639nt和662nt～682nt。设计了对应靶点的反义寡核苷酸A1、A2、A3和10-23型DNAzymeD1、D2和D3。将反义寡核苷酸和Fas基因RNA结合再加入RNaseH进行反应，10-23型DNAzyme则直接与Fas基因RNA作用，结果表明：3个靶点的反义寡核苷酸组及DNAzyme均能降解Fas基因RNA，为有效靶点，其靶点反应优势次序为靶点3〉靶点l〉靶点2。而非靶点对照组和有效靶点突变了2个碱基的对照组均没有反应。靶点2和靶点3与ISIS公司经过多次实验筛选到的Fas反义作用靶点位置基本相同，表明PARASS技术的有效性和可靠性。获得的有效反义寡核苷酸和DNAzyme为后续研究打下基础。

英文摘要：

Three candidate antisense target sites of mouse Fas gene were screened by PARASS （poly-A anchored RNA accessible sites screening） technology. They were target at Fas gene 297nt～317nt, 618nt～ 638nt and 662nt～682nt. Antisense oligos （ A1, A2 and A3 ） and DNAzymes （ D1, D2, and D3 ） for every target site were designed and synthesized. In vitro, the validation of the sites were judged by antisense oligos included RNase H splicing and the DNAzyme degradation. The results indicated that A1, A2 and A3 introduced RNase H degradation. DNAzymes D1, D2 and D3 cleaved Fas mRNA effectively. Neither degradation observed in antisense oligo RNase H group in non-target site （ 1211～1231nt） and 2 bases mismatched of A3, nor splicing occurred in DNzyme group in non-target site （1211～1231nt） and 2 bases mismatched of D3. Site 2 and 3 were at the same positions with those of ISIS Pharmaceuticals. The effective antisense oligos and DNAzymes for Fas gene could be used for the research subsequently.