中文摘要：

采用农杆菌介导法，将BtcrylIa8抗虫基因转入早熟春甘蓝自交系F2011中，共获得37株卡那霉素抗性植株，其中23株经PCR检测呈阳性；Southernblot检测结果表明，crylIa8基因已成功整合到甘蓝基因组中；RT-PCR和Westernblot检测结果表明，crylIa8基因在RNA水平和蛋白质水平均得到表达；对转基因植株进行ELISA检测，其Bt毒蛋白含量在201．9～241．3ng·g^-1（FW）之间；离体饲虫试验结果表明，转基因植株对敏感小菜蛾和CrylAc抗性小菜蛾均具有较好的抗性，且Bt毒蛋白表达量越大，植株抗性越强。

英文摘要：

crylIa8 gene was introduced into inbred line F2011 of early maturing spring cabbage （Brassica oleracea L. vat. capitata L. ） via Agrobacterium tumefaciens-mediated transformation. Thirty-seven transformants with kanamycin-resistance were obtained. Among them 23 plants were positive by PCR analysis. The result of Southern blot analysis demonstrated that crylIa8 gene was integrated into the genome of cabbage. RT-PCR and Western blot detection showed that crylIa8 gene was expressed in RNA and protein levels. The ELISA transgenic plant analysis indicated that its endotoxin protein was 201.9-241.3 ng · g^-1 （ fresh weight ）. The result of in vitro feeding experiment showed that the transgenic plant might control Diamondback moth strains both susceptible to Bt toxin and resistant to CrylAc toxin.