中文摘要：

目的基于黄芪皂苷碱水解生成黄芪甲苷，建立测定不同产地黄芪药材中黄芪甲苷含量的方法。方法色谱柱为ZorbaxEclipseXDB—C18柱（250mm×4．6mm，5μm），流动相为乙腈和水（梯度洗脱），流速为1mL／min，柱温为30℃，ELSD参数为喷雾器温度39℃，漂移管温度75℃，载气压力172．4kPa，增益值为200。结果黄芪甲苷进样量在1．601～9．606μg范围内与峰面积呈良好线性关系（r=0．9991），平均回收率为99．69％，RSD为3．47％（n=6）；山西浑源产的黄芪药材中黄芪甲苷含量最高。结论此方法简便、可行，精密度高、重复性好，能够有效地控制黄芪药材的质量。

英文摘要：

Objective To establish a high performance liquid chromatography- evaporative light- scattering detector（HPLC-ELSD） determination method of astragaloside 1Vcontent in Radix Astragalus from different habitats based on astragalus saponin generating astra- galoside IV by alkali hydrolysis. Methods The chromatographic column was the Zorbax Eclipse XDB-C18 column（250 mm ×4.6 mm, 5 μm） with the mobile phase of CH3CN- H2O, the flow rate was 1 mL/min, the column temperature was 30 ℃, the sprayer temperature was 39 ℃, the drift tube temperature was 75 ℃, the carrier gas pressure was 172.4 kPa and the gain value was 200. Results Astra- galoside IV showed good linearity in the range of 1.601-9.606 μg（r=0.999 1）,the average recovery rate was 99.69% （n=6） and RSD was 3.47%. The astragaloside IVcontent in Radix Astragalus from Shanxi Hunyuan was highest. Conclusion This method is simple and feasible with high precision and good reproducibility, it can effectively control the quality of medicinal material Radix Astragalus.