中文摘要：

目的：建立并评价以CoroNaGreen检测细胞内Na^＋含量变化的流式细胞术方法。方法：完全生长培养基培养的H9c2心肌细胞分别给予Na^＋通道阻断剂TTX（最终作用浓度分别为10、50mmol／L）和Na^＋通道激动剂Veratfidine（最终作用浓度分别为5、10μmol／L），加CoroNaGreen后用流式细胞仪对其信号进行检测；H9c2心肌细胞在含有NaCI（最终作用浓度分别为25、50、100、150mmol／L）的完全生长培养基中培养24h，加CoroNaGreen后用流式细胞仪对其信号进行检测。结果：与阳性对照组相比，等渗状态给予Na^＋通道阻断剂TTX大于10nmol／L时胞内荧光信号强度明显减少（P〈0．05），而Na^＋通道激动剂Veratridine对胞内荧光信号强度无明显影响（P〉0．05）；在高渗状态下，随着NaCl浓度的增高，细胞内荧光信号明显增强（P〈0．05），提示Na^＋内流明显增多。结论：CoroNaGreen作为新型的荧光Na^＋指示剂，与流式细胞术结合，可以简便、有效的检测细胞内游离Na＋含量。

英文摘要：

Objective： To evaluate a flow cytometry based platform for detection of intracellular sodium content by using CoroNa Green, a novel sodium dye. Methods： H9c2 eardiomyoblasts in complete growth medium were treated with Na＋ channel antagonist Trx （final concentrations were 10, 50 nmol/L） and Na＋ channel agonist Veratridine （final concentrations were 5, 10 μmol/L）. After added CoroNa Green, the fluorescence signal was detected by flow cytometry. In addition, the fluorescence signal of H9c2 cells which treated with NaC1 （final concentrations were 25, 50, 100, 150 mmol/L） for 24 h were also detected with CoroNa Green. Results： In isotonic state, intraeellular fluorescent intensity was significantly reduced when TI＇X concentration above 10 nmol/L（P〈0.05 ）, while sodium channel agonist Veratridine did not affect CoroNa Green fluorescent intensity （P〉0.05）. In hyperosmotie state, with the NaC1 concentration increased, the in- traeellular fluorescence signal enhanced significantly （P〈0.05）. Conclusion： CoroNa as a new fluorescent Na＋ indicator can effectively detect intracellular free Na＋ concentration with relative ease.