中文摘要：

根据细胞结构及其电学参数提出了电阻电容电路模型模拟电场对细胞的作用，并利用该模型解释了实验研究中不同斜率的斜坡电场作用后，细胞死亡特性的差异现象。还利用该模型分析了特定斜率的斜坡电场对肿瘤细胞的选择性作用。结果表明由于亚性细胞其线粒体膜上的跨膜电压上升较正党细胞为快，而其细腻膜上的跨膜电压上升则较慢，说明这种选择性是存在的。

英文摘要：

A resistor-capacitor （RC） circuit model is proposed to simulate the effect of applied electric field on cell. The experiment settings are designed to produce pulsed electric field with the intensity peak of 2.12 MV/m within 100 ns and 1 μs, respectively. Cell death rates are assessed after electric stimulation. Cell model which includes mitochondria is used to propose the corresponding RC circuit model and deduce the transmembrane voltage equation. With the experimental parameters and cell structural, cell electric parameters from related papers, transmembrane voltages of cytomembrane and mitochondria membrane both in normal and malignant cells are calculated as the function of time. The result shows that the transmembrane voltage of mitochondria membrane rises much faster than that of cytomembrane after the 100 ns pulsed electric field stimulated. Though the result is reversed if the 1 μs pulsed electric field effected. Apoptosis can be induced when the mitochondria membrane is electroporated and cell will die in another way from that induced by cytomembrane electroporation. The RC circuit model and calculation correspond with the experiment result. Cell death rates are recorded after stimulation with recovery time interval of 30 minutes. Cell death rate of 100 ns pulsed electric field rises slowly and reaches its peak later than that of 1 μs pulsed electric field. It means the 100 ns pulsed electric field causes the death of cell in a more indirectly way contrasting with the 1 μs pulsed electric field. The calculation also shows that the induced transmembrane voltage is different in normal and malignant cell even if they are stimulated by the same pulsed electric field. The requirement of this selectivity is analyzed in the paper.