中文摘要：

目的观察咪达唑仑对七氟烷镇痛和催眠作用的影响。方法将40只小鼠随机均分成四组：生理盐水（NS）组、咪唑安定＋NS组（M组）、NS＋七氟烷组（S组）和咪达唑仑＋七氟烷组（MS组）。实验方法：咪达唑仑2.7 mg/kg腹腔注射;甩尾法、催眠实验中分别腹腔注射七氟烷2.1、3.8 ml/kg;扭体法实验中皮下注射七氟烷3.0 ml/kg。甩尾法记录小鼠尾巴自进入水中到甩出水面的时间（甩尾潜伏期,TFL）;扭体法观察小鼠腹腔注射1%冰醋酸0.1 ml/10 g后,15 min内的扭体次数;催眠实验记录小鼠翻正反射消失至恢复的时间（睡眠时间）。结果与NS组比较,S组、MS组TFL和睡眠时间延长,扭体次数减少（P〈0.05）;与S组比较,MS组TFL和睡眠时间明显延长,扭体次数减少（P〈0.05）。结论咪达唑仑可增强七氟烷的镇痛及催眠效应。

英文摘要：

Objective To observe the effects of midazolam on sevoflurane-induced analgesia and hypnosis in mice.Methods Forty mice were randomly and equally divided into four groups of normal saline（NS）,M（midazolam＋NS）,S（NS＋sevoflurane） and MS（midazolam＋sevoflurane）.Midazolam 2.7 mg/kg was intraperitoneally injected in all tests.Sevoflurane in the doses of 2.1 ml/kg and 3.8 ml/kg was intraperitoneally injected in tail-flick test and hypnosis test,respectively.Sevoflurane 3.0 ml/kg was hypodermically given in twisting test.The time from the tail soaking into to flicking out of the surface of water（TFL） was recorded in tail-flick test,while the times of body twisting were observed after intraperitoneal injection of 1% acetum 0.1 ml/10 g in 15 mimutes in twisting test.The time from disappearance to recovery of righting reflex（sleeping time） was recorded.ResultsCompared with group NS,the TFL and sleeping time were increased,and the times of body twisting decreased in groups of S and MS（P0.05）.In group MS,the TFL and sleeping time were longer than those in group S,while the times of body twisting were less than those in group S（P0.05）.Conclusion Midazolam can enhance the analgesic and hypnotic effects of sevoflurane.