中文摘要：

对双酚A（BPA）暴露下的斑马鱼胚胎、仔鱼和成鱼三个发育阶段进行对比研究，并从代谢和DNA损伤两个角度对其致毒机理进行初步探讨．结果显示：斑马鱼成鱼24h的LC50（8．00-10．00mg·l^-1）比胚胎（16．4±0．40mg·l^-1）低，表明斑马鱼成鱼更适用于急性毒性试验，而胚胎更能体现出亚急性效应和遗传效应；在5．00mg·l^-1的BPA中连续暴露48h后，胚胎和成鱼体内的BPA含量分别为373．57±39．76μg·g^-1和155．78±17．97μg·g^-1，即胚胎在5．00mg·l^-1 BPA溶液中的蓄积能力大约是成鱼的两倍；DNA断裂检测表明BPA对斑马鱼胚胎的DNA造成了一定程度的损伤．

英文摘要：

To evaluate the toxic effects of bisphenol A （BPA）on aquatic organism, different developed stages of zebrafish （embryo, larva and adult fish） were exposed to BPA solution, and the DNA break determination was done on zebrafish embryo. The toxic mechanisms of BPA on zebrafish were discussed primarily. Results showed the 24h LC50 of BPA on adult fish （8.00-10. 00mg·l^-1） was significant lower than that on the embryo （16. 4 ±0. 40mg·l^-1）, indicated adult zebrafish was more suitable for acute experiment while embryos were significant for the studies of sub-acute and genetic toxicity. While exposed to 5.00mg·l^-1 BPA, the BPA contents in embryo and adult fish were 373.57±39. 76μg·g^-1 and 155.78 ± 17. 97μg· g^-1, which suggested that bio-accumulated ability to BPA in embryo was nearly twice of that in adult fish. This study also proved a result of DNA break in zebrafish embryo exposed to a lower BPA solution （5.00mg·l^-1 ） .