中文摘要：

目的 ：研究人疱疹病毒（human herpesvirus,HHV）6B感染T淋巴细胞系Molt3后对其细胞增殖和周期的影响。方法 ：HHV-6B感染Molt3细胞后,倒置显微镜观察细胞形态变化,PCR鉴定Molt3细胞中HHV-6 U22基因,间接免疫荧光及Western blot检测HHV-6蛋白表达;MTT检测细胞增殖,流式细胞仪检测细胞周期变化,荧光定量PCR检测细胞周期相关蛋白m RNA水平变化。结果：HHV-6B感染48 h后,Molt3细胞出现典型细胞病变。PCR检测到感染组细胞中含有HHV-6 U22基因,间接免疫荧光及Western blot均检测到HHV-6蛋白表达。MTT显示HHV-6B能明显抑制Molt3细胞的增殖。HHV-6B感染后Molt3细胞周期发生改变,与对照组相比,感染组细胞G1期增多,S期和G2期减少。实时荧光定量PCR表明HHV-6B感染后cyclin E1m RNA水平降低,p53 m RNA水平升高。结论：HHV-6B能够有效感染Molt3细胞引起典型的细胞病变效应,HHV-6B感染使细胞周期阻滞在G1期从而抑制细胞增殖。

英文摘要：

Objective：To study the significance of human herpesvirus type 6B（HHV-6B） infection on human T-lymphoblastoid cell line Molt3 and its effects on cell cycle and proliferation. Methods：HHV-6B-infected Molt3 cell morphology was assessed by a inverted microscope. The fragment of HHV-6 U22 gene in Molt3 cells was amplified by PCR. Expression of HHV-6B late protein was examined by the immunofluorescence assay and Western Blot. Cell proliferation was measured by MTT assay after HHV-6B infected Molt3. Cell cycle analysis was evaluated by flow cytometry. The m RNA levels of cell cycle related protein were examined by Realtime PCR. Results：Molt3 cells infected by HHV-6B showed typical cytopathic effect at 48 h post-infection. HHV-6 U22 gene was detected by PCR. HHV-6 late protein was positive in infected cells by indirect immunofluorescence and Western Blot assay. MTT assay detected that HHV-6B infection inhibited the proliferation of Molt3 cells significantly. Compared with the uninfected cells,percentages of Molt3 cells infected by HHV-6B were increased in phase G1 but decreased in phase S and G2. The m RNA levels of cyclin E1 were decreased after 48 h post-infection,however,the m RNA levels of p53 were significantly increased after infection.Conclusion：HHV-6B can infect the Molt3 cells,lead to typical cytopathic effect,and inhibit cell proliferation and make the cell cycle stay in phase G1.