中文摘要：

背景气喘是长期的航线疾病，发炎由生理的变化描绘了(航线 hyper-responsiveness， AHR ) 并且病理学的变化(煽动性的房间渗入和粘液生产) 。嗜曙红血球在过敏发炎起一个关键作用。但是在嗜曙红血球和航线发炎之间的原因的关系是难的证明。原因之一是鼠科的嗜曙红血球的激活标记的缺乏。我们在 vitro 在鼠科的嗜曙红血球上调查了表示 ofCD69，在从外部血和 bronchoalveolar 洗室年龄液体的嗜曙红血球上并且在在气喘的老鼠的航线发炎上的 CD69 的表示之间的关系。从 IL-5 转基因的老鼠(NJ.1638 ) 的外部血的方法嗜曙红血球被净化。划分成五个组的 Micewere：敏化并且质问与的野类型老鼠盐( WS 组)，与 ovalbumin ( WO 组)敏化并且质问的野类型老鼠，IL-5~(敏化并且质问与的-/-)老鼠与净化的嗜曙红血球( ISE 组)盐、转移，IL-5~(与卵敏化并且质问并且与净化的嗜曙红血球( IOE 组)转了的-/-)老鼠，IL-5~(与卵敏化并且质问并且与净化的嗜曙红血球转了的 -/-)mice ，有反 CD4 单音的同种细胞的抗体( IOE+antiCD4mAb 组)的 pretreated 。IL-5~(-/-) 老鼠在 day0 和天与卵被敏化 14，然后与卵质问了喷雾器。在天， 24， 25， 26 和 27 净化了嗜曙红血球我们 retransferred intratracheally 到 IL-5~(-/-) 老鼠。在白天 28 上，血和 BALF 被收集，嗜曙红血球上的 CD69expression 由 flowcytometry 测量了。结果净化了嗜曙红血球 expressCD69。但是与 PMA+MA， IFN- γ， IL-5 或 GM-CSF 有教养的嗜曙红血球强烈表示了 CD69。从 WO 的血的嗜曙红血球， WS 组根本没表示 CD69。WO 组， IOE 组， ISE 组和 IOE+antiCD4mAb 组的嗜曙红血球 inBALF 的数字比在根本没有嗜曙红血球的 WS 组的老鼠显著地高。在 BALF ofIOE 和 WO 组的嗜曙红血球上的 CD69 表示是强壮的。在 ISE 和 IOE+antiCDmAb 组的 BALF 的嗜曙红血球 expressCD69。粘液生产结果类似于 CD69 表?

英文摘要：

Background Asthma is a chronic airway disease with inflammation characterized by physiological changes （airway hyper-responsiveness, AHR） and pathological changes （inflammatory cells infiltration and mucus production）. Eosinophils play a key role in the allergic inflammation. But the causative relationship between eosinophils and airway inflammation is hard to prove. One of the reasons is lack of activation marker of murine eosinophils. We investigated the expression of CD69 on murine eosinophils in vitro, the relationship between the expression of CD69 on eosinophils from peripheral blood and bronchoalveolar lavage fluid and on airway inflammation in asthmatic mice. Methods Eosinophils from peripheral blood of IL-5 transgenic mice （NJ.1638） were purified. Mice were divided into five groups： wild type mice sensitized and challenged with saline （WS group）, wild type mice sensitized and challenged with ovalbumin （WO group）, IL-5^-/- mice sensitized and challenged with saline and transferred with purified eosinophils （ISE group）, IL-5^-/- mice sensitized and challenged with OVA and transferred with purified eosinophils （IOE group）, IL-5^-/- mice sensitized and challenged with OVA and transferred with purified eosinophils, pretreated with anti CD4 monoclonal antibody （IOE＋antiCD4mAb group）. IL-5^-/- mice were sensitized with OVA at day 0 and day 14, then challenged with OVA aerosol. On days 24, 25, 26 and 27 purified eosinophils were transferred intratracheally to IL-5^-/- mice. On day 28, blood and BALF were collected and CD69 expression on eosinophils measured by flowcytometry. Results Purified eosinophils did not express CD69. But eosinophils cultured with PMA＋MA, IFN- T, IL-5 or GM-CSF expressed CD69 strongly. Eosinophils from blood of WO, WS group did not express CD69 at all. The numbers of eosinophils in BALF of WO group, IOE group, ISE group and IOE＋antiCD4mAb group were significantly higher than in mice of WS group which did not have eosinophils at all. CD69 express