中文摘要：

研究不同偶联率的SW-HSA对小鼠免疫原性的影响,为获得理想的SW人工抗原奠定基础。首先将SW与活性酯在70℃条件下反应制备季铵盐,然后将季铵盐与HSA冰浴搅拌反应12 h,通过调节季铵盐与HSA的摩尔比制备SW-HSA,透析并冷冻干燥,采用紫外分光光度法测定SW-HSA偶联率。将20只昆系小白鼠随机分为A、B、C、D和E组,每组4只。用4种不同偶联率的SW-HSA对前4组小鼠进行免疫,首次免疫抗原量为0.050 mg/只,第30天进行第2次免疫,抗原量与首免相同,以后每隔20 d进行1次,抗原量均为前一次免疫所用抗原量的1.5倍,E组为对照,注射等量生理盐水。从第3次免疫后ELISA检测血清中抗SW抗体效价。紫外光谱测定结果显示,合成的SW-HSA偶联率分别为18.6、12.5、13.5和32.6。ELISA测定结果显示,从第3次免疫后,A、B、C、D组试验小鼠血清的抗SW抗体效价均呈上升趋势,在第5次免疫后,测得其抗SW抗体效价分别为28、29、29、29,达到最高峰。其中D组的抗SW抗体效价在第4次免疫后首先达到29,并在第5次免疫后维持在相同水平。各组试验小鼠血清的抗SW抗体效价于第6次免疫时开始逐渐下降。试验结果证实,不同偶联率的SW-HSA均可诱导小鼠产生抗体,较大偶联率的SW-HSA可以更好地提高抗SW抗体水平。

英文摘要：

The immunogenicity of 4 different swainsonine-HSA was studied on mice to explore the condition for obtaining ideal artificial antigen of swainsonine. At first, quaternary ammonium salt was prepared by reaction between SW and active ester at 70℃, and then, SW-HSA was obtained by stirring between quaternary ammonium salt and HSA at condition of ice bath for 12 h, and adjusted mole ratio between quaternary ammonium salt and HSA. Dialyzing and freeze drying the conjugate, SWHSA was assayed by ultraviolet spectrophotometry to detect the conjugate ratio. Twenty kunming mice were randomly separated into group A, group B, group C, group D and group E, 4 mice per groupo Mice of group A, group B, group C and group D were immunized by SW-HSA with 4 kinds of conjugate rates. The dose of antigen of the first immunization was 0. 050 mg per mouse. The second immunization was done at 30 d and the dose of antigen was the same as the first immunization. There was an immunization per 20 d after the second immunization, the dose of antigen was 1.5 times as the dose of antigen before it. G-roup E was control and injected with physiologic saline. Serum SW anti-body titers in each group were assayed initially at the third immunization. The results of ultraviolet spectra assay showed that the conjugate ratio of SW-HSA were 18.6, 12.5, 13.5 and 32.6 respectively. ELISA assay showed that serum SW antibody titers in group A, group B, group C and group D began to increase after the third immunization. Among the groups, SW antibody titers of group A, group B, group C, group D increased to the peak （2^8 , 2^9 , 2^9 , 2^9 respectively） at the fifth immunization. Meanwhile, SW antibody titer of group D was up to 29 at the fourth immunization, and kept the same antibody level at the fifth immunization. SW antibody titers of 4 groups began to decrease gradually at the sixth immunization. The results of this experiment confirmed the SW antibody could generate in the body of mice with 4 different SW-HSA, and the high conjugate ratio could ind