中文摘要：

Coxsackievirus A16 (CVA16 ) 是在孩子的手，脚和嘴疾病(HFMD ) 的主要病原体之一。长非编码的 RNA (IncRNAs ) 在各种各样的生物过程被含有，但是他们没与 CVA16 感染被联系。在这研究，我们包括地描绘了正常的 IncRNAs 的风景， CVA16 感染了 rhabdomyosarcoma (RD ) 调查 IncRNAs 的功能的关联的房间使用 RNA-Seq。我们证明 760 IncRNAs 的一个总数是 upregulated， 1210 IncRNAs 是 downregulated。从这些 dysregulated IncRNAs， 43.64% 是 intergenic， 22.31% 是感觉， 15.89% 是 intronic， 8.67% 是双向的， 5.59% 是 antisense， 3.85% 是 sRNA 主人 IncRNAs， 0.05% 是 enhancer。六 dysregulated IncRNAs 被量的 PCR 试金验证，这些 IncRNAs 的第二等的结构被投射。而且，我们进行了 IncRNAs (ENST00000602478 ) 的生物信息学分析阐明修正的差异和 IncRNAs 的功能。在摘要，当前的学习在 CVA16 挑战之上比较了 dysregulated IncRNAs 侧面并且说明了在编码和 IncRNAs 抄本之间的复杂关系。这些结果不能仅仅在 CVA16 提供抄写的一幅完全的图画感染的房间而且为 HFMD 的处理提供新奇分子的目标。

英文摘要：

Coxsackievirus A16（CVA16） is one of major pathogens of hand, foot and mouth disease（HFMD） in children. Long non-coding RNAs（Inc RNAs） have been implicated in various biological processes,but they have not been associated with CVA16 infection. In this study, we comprehensively characterized the landscape of Inc RNAs of normal and CVA16 infected rhabdomyosarcoma（RD）cells using RNA-Seq to investigate the functional relevance of Inc RNAs. We showed that a total of 760 Inc RNAs were upregulated and 1210 Inc RNAs were downregulated. Out of these dysregulated Inc RNAs, 43.64% were intergenic, 22.31% were sense, 15.89% were intronic, 8.67% were bidirectional, 5.59% were antisense, 3.85% were s RNA host Inc RNAs and 0.05% were enhancer. Six dysregulated Inc RNAs were validated by quantitative PCR assays and the secondary structures of these Inc RNAs were projected. Moreover, we conducted a bioinformatics analysis of an Inc RNAs（ENST00000602478） to elucidate the diversity of modification and functions of Inc RNAs. In summary, the current study compared the dysregulated Inc RNAs profile upon CVA16 challenge and illustrated the intricate relationship between coding and Inc RNAs transcripts. These results may not only provide a complete picture of transcription in CVA16 infected cells but also provide novel molecular targets for treatments of HFMD.