中文摘要：

背景：钛磨损颗粒在人工关节假体周围骨溶解的形成中具有重要作用，其对成骨细胞的影响可能是骨溶解形成的原因之一。目的：舰察钛颗粒对大鼠成骨细胞增殖、分化及矿化功能的影响。设计、时间及地点：随机分组设计，对比观察，于2008—09／12存上海交通大学医学院附属第九人民医院骨科实验室完成。材料：商品化纯钛颗粒（货号：00681：JohnsonMatthey，WardHill，MA，USA），平均粒径4．5Um，86％的颗粒小于10μm。方法：分离培养新生24h内SD大鼠成骨细胞，选用第3代大鼠成骨细胞接种于培养皿／板中，24h后细胞完_全贴譬，更换无血清培养液孵育24h，根据是否加入钛颗粒条什培养基分为钛颗粒组和对照组，其后钛颗粒组更换含01g／L钛颗粒的条件培养基，3d换液1次。对照组同时换液，但不加入含钛颗粒的条件培养基。主要观察指标：于2，4，7，14d采用四甲基偶氮唑盐法榆测细胞增殖情况；第7天和第14天行碱性磷酸酶染色和碱性磷酸酶定量观察细胞的碱性磷酸酶活性变化；第14，21天行茜素红钙结节染色观察细胞矿化能力的变化。结果：钛颗粒组与对照组相比各时间点细胞增殖无明显差异（P〉0.05）；7d和14d碱性磷酸酶染色可见钛颗粒组较对照组减弱，碱性磷酸酶活性定量分析钛颗粒组明显低于对照组（P〈0．01）；14，21d茜素红染色可见钛颗粒组钙结节数量较对照组明显降低。结论：铁颗粒对大鼠成骨细胞分化和矿化功能具有抑制作用，但不影响细胞增殖。

英文摘要：

BACKGROUND： Titanium particles play an important role in the formation of periprosthetic osteolysis, and the effect of titanium particles on osteoblasts maybe the reason for osteolysis. OBJECTIVE： To observe the effect of titanium particles on the cell proliferation, differentiation and mineralization of osteoblasts. DESIGN, TIME AND SETTING： The randomized grouping control experiment was performed at the Department of Orthopedic Surgery, Shanghai Ninth People＇s Hospital, Shanghai Jiao Tong University School of Medicine from September to December 2008. MATERIALS： The commercial pure titanium particles （number 00681, a product made by Johnson Matthey, Ward Hill, MA, USA） with mean diameter of 4.5 μm, and 86% of which shorter than 10 pro. METHODS： Osteoblasts of new born SD rats within 24 hours were insolated, and cells of the third passages were inoculated at culture plate, replace medium when cells attached completely at 24 hours after culture. Then cells were divided into control and experimental groups. Osteoblasts in the experimental group were cultured with titanium particles at a concentration of 0.1 g/L, and medium was renewed once in 3 days. The medium in the control group was renewed simultaneously. MAIN OUTCOME MEASURES： MTT test was used to assess the cell proliferation at 2, 4, 7, 14 days; while alkaline phosphatase （ALP） staining and ALP activity measurement were taken to assess the differentiation of osteoblasts at days 7 and 14. The mineralization of osteoblasts was evaluated by Alizarin red staining at days 14 and 21. RESULTS： The osteoblastic proliferation had no significant difference between the experimental group and the control group （P 〉 0.05）. However, the ALP staining was weaken in the experimental group than that of control group at days 7 and 14, meanwhile, activity of ALP measurement in the experimental group was smaller than the control group （P 〈 0.01 ）, while the alizarin red staining demonstrated that the quantity of calcium nodules were markedly