中文摘要：

目的构建针对has—miR-146a的慢病毒表达载体，并鉴定成熟has—miR-146a在细胞内表达水平。方法PCR扩增pri-miR-146a，克隆于慢病毒载体plenti—GFP中，转染293FT细胞，收获并浓缩慢病毒颗粒，感染Jurkat细胞。结果成功构建了has—miR-146a的慢病毒表达载体，建立了高效转染系统。结论建立了高效稳定表达has—miR-146a的慢病毒转染系统。

英文摘要：

Objective To construct a lentivirus vector expressing microRNA （miRNA） miR-146a and transfect the vector into Jurkat cell line. Methods PrimiR-146a amplified by PCR was inserted into plenti-GFP vector, and then identified by restriction endonuclease digestion and nucleotide sequencing. Jurkat cell line was transfected with the plasmid pluG-miR-146a. The expression of miR-146a was detected by fluorescence microscopy, flow cytometry, and Real-time PCR. Results The sequence of miR-146a was contained in the constructed recombinant plasmid. After transfection with the plasmid, miR-146a could be expressed in Jurkat cell line. Conclusion The constructed lentivirus vector can express miR-146a in vitro, which is the basis for further study on the function of miR-146a.