中文摘要：

目的：研究参附注射液对心力衰竭大鼠心肌凋亡和凋亡相关蛋白的影响。方法：45只Wistar大鼠随机分为对照组、模型组和治疗组,采用左冠状动脉结扎法建立心力衰竭模型。对照组和模型组给予0.9%氯化钠溶液、参附组给予腹腔注射参附注射液（6.2mL.kg-1.d-1,4周）。采用TUNEL和免疫组化检测心肌凋亡和相关蛋白。结果：共有38只大鼠存活。对照组（n=14）未见心肌凋亡,模型组（n=13）凋亡数量增加,而参附组（n=11）凋亡数量明显减少,与模型组比较差异具有显著性（P〈0.01）。与对照组相比,模型组Bax、Bcl-2、Fas和FasL蛋白表达增加（P〈0.05）;与模型组相比,治疗组Bax、Fas和FasL蛋白表达显著下降（P〈0.01）。结论：参附注射液能够抑制实验性心力衰竭大鼠心肌凋亡,其作用机制与下调促凋亡基因Bax、Fas和FasL表达的作用相关。

英文摘要：

Objective： To investigate the intervention of Shenfu Injection on cardiomyocyte apoptosis and expressions of apoptosis-related proteins in heart failure rats.Methods： Forty-five Wistar rats were randomized into 3 groups： sham,heart failure model and Shenfu Injection.Heart failure was introduced by ligation of the left anterior descending coronary artery.38 rats survived,and animals in the sham group（n=14） and model group（n=13） received a saline injection while animals in the Shenfu Injection group（n=11） received an Injection of Shenfu Injection（6.2mL.kg-1.d-1 ip,4w）.Myocardial cells were measured by TdT-mediated dUTP nick end labeling（TUNEL）,and the expressions of Bax,Bcl-2,Fas and FasL in cardiomyocytes were detected by immunohistochemical assays.Results： The TUNEL stained cardiomyocytes significantly increased,and the expressions of Bcl-2,Fas,FasL as well as Bax in cardiomyocytes significantly up-regulated in model group compared with those in sham rats（P0.05）.Compared with the model group,it was in the Shenfu Injection group that expressions of Bax,Fas,FasL and TUNEL stained cardiomyocytes significantly down-regulated after 4-week Shenfu Injection therapy（P0.01）.Conclusion： Shenfu Injection could Inhibit cardiomyocyte apoptosis in experimental heart failure rats by down-regulating expressions of Bax,Fas and FasL.