中文摘要：

【目的】研究miR-133a在鸡不同组织中的表达情况,对baculoviral IAP repeat containing 5（BIRC5）进行靶基因的试验验证,探讨鸡miR-133a对BIRC5的靶向调控作用。【方法】采用生物信息学方法对miR-133a靶基因进行预测,并用双荧光素酶报告系统及点突变试验对BIRC5进行靶基因验证,同时运用实时荧光定量PCR检测miR-133a在鸡不同组织中的表达量。【结果】在鸡3′UTR数据库的11 891个基因中预测到287个miR-133a的靶基因;miR-133a在鸡的组织表达谱中显示其在肌肉中的表达量较高,尤其是在骨骼肌中表达量最高;报告基因试验显示BIRC5是miR-133a的靶基因,点突变试验证实了miR-133a在BIRC5上结合的靶位点。【结论】miR-133a是与鸡骨骼肌发育高度相关的miRNA,且鸡BIRC5基因是miR-133a的靶基因。

英文摘要：

[Objective] This study was conducted to investigate the expression pattern ofmiR-133a in various chicken tissues, and find whether baculoviral lAP repeat containing 5 （BIRC5） is the target gene ofmiR-133a. [Method] The bioinformatics methods were used to predict the target genes of miR-133a. Real-time PCR was used to detect the expression patterns of miR-133a of various tissues in chicken. Dual-luciferase reporter assays and site mutation assays were used to verify whether BIRC5 is a target gene of miR-133a. [Result] Two hundred and eighty-seven genes of 11891 genes fi＇om 3＇UTR database were predicted as target genes of miR-133a. The expression patterns of miR-133a in various chicken tissues showed that miR-133a was highly expressed in muscle especially in skeletal muscle. Reporter assays showed that BIRC5 was the target gene of miR-133a and the site mutation assays validated the target site ofmiR-133a in BIRC5. [Conclusion] miR-133a is a miRNA related with skeletal muscle development of chicken, and BIRC5 is a bona fide target gene in chicken.