中文摘要：

采用MaxVisionTM免疫组织化学方法,以兔抗人GnRH受体的多克隆抗体,对雄性三疣梭子蟹（Portunus trituberculatus）的神经器官（视神经节、脑神经节和胸神经团）进行免疫组织化学定位.结果显示：GnRH受体的免疫阳性物质在雄性三疣梭子蟹视神经节、脑神经节和胸神经团的多个部位都有较为广泛的存在,定位在神经细胞的细胞质和神经髓质中,不同神经器官GnRH受体的免疫阳性强度有一定的差异.三疣梭子蟹神经器官存在GnRH受体的免疫阳性物质,为GnRH参与其神经调节作用提供了形态学依据.

英文摘要：

The immunohistochemical technique of MaxVisionTM method and human GnRH receptor antibody against rabbit were used in this study to find out the locations of immunoreactive substances in the nervous organs of the male swimming crab,Portunus trituberculatus.The results revealed that：the immunoreactive substances of GnRH receptor were widely detected in many parts of the optic ganglion,cerebral ganglion and thoracic ganglion mass of the male crab.The immunoreactive substances located in the cytoplasm of neurosecretory cells and the neuropils.In addition,there were differentions in the intensity of immunoreaction in these three nervous organs.The immunoreactive substances of GnRH receptor existed in the nervous organs could provide morphologic evidence for GnRH participating in the neuroendocrine regulation in the swimming crab.