中文摘要：

目的探讨抗炎素-1（Antiflammin-1，AF-1）对脂多糖（LPS）诱导的RAW264．7细胞白细胞介素10（interleuki-10，IL-10）表达的影响。方法采用体外培养巨噬细胞RAW264．7细胞，实验分为正常对照组，LPS组和不同浓度的LPS＋AF-1组。应用逆转录PCR（RT—PCR）技术检测IL-10表达的变化。结果1μg／ml的LPS刺激RAW264．7细胞后IL-10的表达较正常对照组增加（P〈0．05），LPS＋AF-1组IL-10表达较LPS组表达显著升高（P〈0．05），并具有剂量依赖性结论AF-1可促进LPS诱导的RAW264．7细胞IL-10表达的增加。

英文摘要：

Objective To investigate effects of Antiflammin- 1 （AF- 1 ） on expression of IL- 10 of RAW264.7 cells induced by endotoxin. Methods A macrophage cell line RAW264.7 was employed in an in vitro experiment. LPS and different concentration of AF- 1 were added respectively after RAW264.7 cells were cultured. The interleukin- 10（IL- 10）mRNA levels in supematant was detected by RT- PCR. Results IL- 10 mRNA levels were higher in groups stimulated by 1 μg/ml LPS than those in the blank control group（ P 〈 0.05）. AF- 1 could significantly increased IL - 10 expressions in an concentration - dependently（ P 〈 0.05 and P 〈 0.01 ）. Conclusion AF- 1 could significantly mRNA increased IL- 10 expressions of RAW264.7 cells induced by LPS.