中文摘要：

目的探讨不同类型淋巴瘤患者肿瘤坏死因子-β（TNF-β）在肿瘤组织与血浆中表达及其临床意义。方法收集97例不同类型淋巴瘤患者石蜡组织和血浆标本，应用免疫组化和ELISA法观察组织与血浆中TNF-β表达情况。结果TNF-β在霍奇金淋巴瘤（HL）、弥漫大B细胞淋巴瘤（DLBCL）、浆细胞肿瘤（PCN）、小淋巴细胞淋巴瘤／慢性淋巴细胞白血病（SLL／CI，L）、滤泡性淋巴瘤（FL）、周围T细胞淋巴瘤（PTL）表达较高，而在淋巴母细胞淋巴瘤（LBL）、结外NK／T细胞淋巴瘤（NK／TCL）表达较低，各种类型淋巴瘤组织TNF-β的表达情况比较，差异无统计学意义（P〉0．05）。在HL、DLBCL、PCN、SLL／CLL、FL、PTL患者血浆中TNF-β水平高于健康对照组，差异有统计学意义（P＜0．05）。组织中TNF-~表达在国际预后指标（IPI）评分的中、高危组与低危组比较，差异有统计学意义（P＜0．05）。TNF一口在组织与血浆中的表达无相关性（rs=0．12，P〉0．05）。结论TNF-β在淋巴瘤组织与血浆中都有不同程度表达，提示TNF-β在淋巴瘤发生、发展中起到一定作用，但是TNF-β与淋巴瘤的关系比较复杂，不同的类型可能存在不同的机制。

英文摘要：

Objective To investigate the expression of tumor necrosis factor β（TNF-β） in tissue and plasma of subtypes of lym- phoma and its significance. Methods Immunohistochemical technique and enzyme-linked immunosorbent assay（ELISA） were re- spectively used to assess the expression of TNF-β in 97 cases paraffin tissue and plasma samples of subtypes of lymphoma. Results Expressions of TNF-β were higher in HL, DLBCL, PCN, SLL/CLL, FL and PTL then in LBL, and NK/TCL. Expressions of TNF-β in tissue of subtypes of lymphoma had not significant difference（P〉0.05）. Expression of TNF-β in plasma of HL,DLBCL, PCN, SLL/CLL,FL and PTL were higher than control group,TNF-β in these lymphoma were significant difference（P〈0.05）. Ex- pressions of TNF-β in tissue had significant difference between low-risk group and middle or high risk group of international prog- nosis index（P〈0.05）. Expressions of TNF-β in tissue and plasma of subtypes of lymphoma had no correlation（rs= 0. 12, P〉 0.05）. Conclusion TNF-β has been found various degree expressions in tissue and plasma of lymphoma,TNF-β play a role in the development of lymphoma, but the expression of TNF-β in subtypes of lymphoma may exist different mechanisms.