中文摘要：

研究了在生物医学领域广泛应用的Fe3O4纳米颗粒与多种蛋白（包括白蛋白、纤维蛋白和免疫球蛋白等）的相互作用及其生物学效应，结果发现Fe3O4纳米颗粒与蛋白的相互作用存在蛋白种类选择性，对蛋白的吸附能力由强到弱依次为纤维蛋白〉免疫球蛋白〉白蛋白。形成的Fe3O4纳米颗粒一蛋白复合物有效地降低了纳米颗粒的细胞毒性。

英文摘要：

The aim is to study theFe3O4 nanoparticle-protein interactions and their bioeffects. Methods： Transmission electron microscope （TEM）, light microscopy, SDS-PAGE （Sodium Dodecyl Sulfonate-Polyacrylamide Gelelectrophoresis）, Bradford and MTT （3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide） methods were used to investigate the Fe3O4 nanoparticle-protein interactions and their bioeffects. Results： The adsorption capacities of Fe3O4 nanoparticle for different proteins were different, and the order was fibrinogen〉immunoglobulin〉albumin. The adsorption of proteins on the Fe3O4 surface resulted in much reduced cytotoxicity for these protein-coated Fe3O4 nanoparticles. Conclusion： These findings suggest that we should take the Fe3O4 nanoparticle-protein interactions into consideration when designing safe Fe3O4 nanoparticles for biomedical applications.