中文摘要：

本研究探讨HLA-DQB1＊02、03等位基因及细胞因子IFN-γ和IL-4与广西壮、瑶族肝癌家庭聚集性的相关性。将来自肝癌高发区，民族为瑶族的肝癌高发家族成员、无癌家族成员各40例及民族为壮族的肝癌高发家族成员、无癌家族成员各48例作为研究对象（采用相同性别，年龄（±5岁）配对方法），采集研究对象外周血并提取全血DNA，采用PCR-SSP技术检测受试者的HLA-DQB1＊02和HLA-DQB1＊03等位基因，IFN-γ及IL-4细胞因子表达水平采用ELISA法检测。结果发现，HLA-DQB1＊02、03基因在壮族肝癌高发家族成员组的表达频率均低于无癌家族成员组（p〈0．05）；HLA-DQB1＊02基因在瑶族肝癌高发家族成员组的表达频率高于无癌家族组（p=0．002），HLA-DQB1＊03在两组间的表达频率无明显差异（p〉0．05）。广西壮、瑶族人群HLA-DQB1＊02、03基因在乙肝病毒感染组及非感染组中的表达频率比较无显著性差异（p〉0．05）。壮、瑶族肝癌高发家族成员组IL-4平均表达水平均高于无癌家族成员组，肝癌高发家族成员组IFN-γ平均表达水平均低于无癌家族成员组，差异具有统计学意义（p〈0．05）。广西壮、瑶族人群中HLA-DQB1＊02基因阳性和阴性成员的IL-4平均表达水平无明显差异（p〉0．05）。广西壮族人群中HLA-DQB1＊03基因阳性成员的IFN-γ平均表达水平高于HLA-DQB1＊03阴性成员（p=0．011），HLA-DQB1＊03阳性和阴性成员的IL-4平均表达水平无明显差异（p〈0．05）。本课题得出结论，HLA-DQB1＊02、03基因与广西壮、瑶族肝癌家庭聚集性有关，但存在较大的民族差异；细胞因子IFN-γ及IL-4的表达水平失衡可能是广西壮、瑶族肝癌家族聚集性的危险因素。HLA-DQB1＊03基因导致广西壮族肝癌家族聚集性的发生可能是通过影响IFN-γ的表达水平来实现的。

英文摘要：

This study aimed to investigate the relationship between HLA -DQBl＊02,03 alleles as well as IFN-γ and IL-4 cytokines and familial aggregation of hepatocellular carcinoma （HCC） in Guangxi Zhuang and Yao people. From the high incidence areas of HCC, we selected 40 Yao members from high liver cancer incidence family, 40 people of Yao from no-cancer families, 48 members of Zhuang from high liver cancer incidence family, and 48 people of Zhuang from no-cancer families as study objects （with the same gender, age （±5） year pairing method）. The sample of peripheral blood was collected and the whole blood DNA were extracted, the detection of HLA-DQB1＊02 and HLA-DQB1＊03 alleles was made by PCR-SSP technology, and the expression levels of IFN-γ and IL-4 cytokines were detected by ELISA method. The results showed that the expression frequency of HLA-DQB1＊02, 03 genes in the high liver cancer incidence family group of Zhuang was lower than that in no-cancer family group （p〈0.05）, while the expression frequency of the HLA -DQB1＊02 gene in the high liver cancer incidence family group of Yao was higher than that in no-cancer family group （p=0.02）, but the expression frequency of HLA-DQB1＊03 gene had no significant difference between those two groups （p〉0.05）. The expression frequency of HLA-DQBI＊02, 03 gene from Guangxi Zhuang and Yao people in hepatitis B virus infection group and non-infection group was of no significant difference （p 〉0.05）. The average expression level of IL-4 in the high liver cancer incidence family group was higher than that in no-cancer family group, but the expression level of IFN-γ in the high liver cancer incidence family group was lower than that in no-cancer family group, and the difference was statistically significant （p〈0.05）. There was no significant difference between the expression levels of IL-4 in Zhuang and Yao people with positive and negative HLA-DQB1＊02 （19 〉0.05）. The average expression level of IFN-γ in Zhuang people wi