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双向等位基因特异性PCR技术在杏SNP分型上的应用
  • ISSN号:1009-9980
  • 期刊名称:果树学报
  • 时间:2013
  • 页码:933-937
  • 分类:S662.2[农业科学—果树学;农业科学—园艺学]
  • 作者机构:[1]辽宁省果树科学研究所,辽宁熊岳115009
  • 相关基金:基金项目:国家自然科学基金(31071767);国家公益性行业(农业)科研专项(201003058-2);农业部国家种质资源保护项目(NB2012-2130135-03)
  • 相关项目:杏果实硬度的关联分析与功能标记研究
中文摘要:

【目的】种质资源的遗传多样性研究是资源工作的核心,了解和掌握种质资源资源多样性,对于资源收集、管理、发掘、利用方面具有重要意义。【方法】对219份普通杏种质资源的11个性状进行了相关分析、主成分分析和聚类分析。【结果】普通杏种质资源各性状变异系数的变幅为15.7%。42.2%,其中可溶性固形物含量的变异系数最小,平均单果质量的变异系数最大。相关性分析得出:平均单果质量与叶柄长度、叶片长度和叶片宽度呈极显著正相关;可溶性固形物含量与可溶性糖呈极显著正相关:维生素C含量与可溶性固形物含量呈极显著正相关.与1a生枝粗度呈显著负相关。主成分分析结果表明:11个性状可以综合为4个主成分,前4个主成分累计贡献率达58.65%.第1主成分反映叶片因子,第2主成分反映枝条因子,第3、第4主成分反映的是品质因子。根据聚类分析把219份杏种质资源划分为6大类群:第1类种质群的主要特点是叶片较大:第Ⅱ类种质群的特点是果实品质性状较好.但是果实平均单果质量小;第Ⅲ类种质群的特点是果实平均单果质量大:第V类种质群的特点是果实品质好:第Ⅵ类种质群的特点是叶片较大且1a生枝又粗又长。【结论]普通杏种质资源的主要数量性状的变异较大,遗传多样性较丰富。

英文摘要:

[Objective]Genetic resource evaluation is an important part of research work. Understanding and mastering the diversity of germplasm resources make a significant contribution to collection, manag- ment, exploration and utilization the resource. [Method]The 219 common apricot materials were evaluat- ed based on correlation analysis,principal component analysis (PCA)and cluster analysis. [Result]The variation coefficients of the traits assayed were from 15.7% to 42.2% ,among them soluble solid content presents the smallest coefficient of variation, while average fruit weight the largest. The correlation analy- sis showed that there were significantly positive correlations between average fruit weight and both leaf stalk length, leaf length and leaf width, and between soluble solid content and both soluble sugar content and vitamin C content, and there were significantly negative correlation between vitamin C content and branch diameter. The principal components analysis showed that the cumulative contribution of variance of the first four traits principal components accounted for 58.65%, the first one reflected leaf characters, the second branch characters, the third and the fourth fruit quality characters. The clustering results showed that the main trait of the first group is large leaf; the second one is better fruit quality but the average fruit weight is small; the third one is large average fruit group is large leaf and long, thick annual branches. weight; the fifth group is good fruit quality; the sixth [Conclusion]The main quantitative traits of common apricot germplasm resources in China have extensive variation with rich genetic diversity

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期刊信息
  • 《果树学报》
  • 北大核心期刊(2011版)
  • 主管单位:农业部
  • 主办单位:中国农业科学院郑州果树研究所
  • 主编:王志强
  • 地址:河南省郑州市航海东路南中国农业科学院郑州果树研究所
  • 邮编:450009
  • 邮箱:chinagsxb@163.con
  • 电话:0371-65330928 653387308
  • 国际标准刊号:ISSN:1009-9980
  • 国内统一刊号:ISSN:41-1308/S
  • 邮发代号:36-93
  • 获奖情况:
  • 获河南省优秀期刊二等奖
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,美国剑桥科学文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:18699