用多分子的标记技术并且基于词法标准,在 Epinepheelus malabaricus 和 E 之间的基因关系。coioides 被检验希望解决识别这的长期的问题二种。结果显示出那:(1 ) E。coioides 和 E。malabaricus 应该作为二种被识别,在 E 之间的一致性 ofmitochondrial DNA 细胞色素 b 基因顺序。malabaricus 和 E。coioides 是 94.4% ,并且由 AFLP 的基因类似是 0。753 9;(2 ) 杂交在 E 之间存在。malabaricus 和 E.coioides,特定的 RAPD 和 AFLP 碎片被发现在这的鉴定有用二种,和基因性质(与外表和继承) 混血儿显著地被偏导到男父母;并且(3 ) AFLP 是在为这二组 ers 构造基因连接地图的一个潜在地强大的工具。
Using multi-molecular marker technologies and based on morphological criteria, the genetic relationship between Epinepheelus malabaricus and E, coioides was examined in the hope of resolving the long-standing issue of identifying these two species. Results showed that: ( 1 ) E. coioides and E. malabaricus should be identified as two species, the consistency of mitochondrial DNA cytochrome b gene sequence between E. malabaricus and E. coioides is 94.4%, and the genetic similarity by AFLP was 0.753 9 ; (2) Hybridization exists between E. malabaricus and E. coioides, the specific RAPD and AFLP fragments are found to be useful in the identification of these two species, and the genetic properties (both with exterior and inheritance) of hybrid is significantly biased to the male parents ; and (3) AFLP was a potentially powerful tool in constructing the genetic linkage map for these two groupers.