中文摘要：

目的：探讨牙周炎时SDF-1α/CXCR4轴对PDLSCs血管内皮向分化的影响。方法：分别从正常和炎症牙周组织分离培养鉴定牙周膜干细胞（H-PDLSCs、P—PDLSCs）；RealtimePCR检测两种干细胞中基质细胞衍生因子-1α（stromalcell—derivedfactor-1α，SDF-1α）及趋化因子受体4[chemokine（c—x—cmotif）receptor4，CXCR4]的表达；然后用SDF-1α分别对两种干细胞进行诱导（14d），RealtimePCR检测H-PDLSCs和P—PDLSCs中成血管相关基因CD51、VwFmRNA的表达；Matrigel基质胶管腔形成实验观察、比较H-PDLSCs和P—PDLSCs的管腔形成情况及差异。结果：P—PDLSCs较H—PDLSCs高表达CXCR4 mRNA（P〈0．05），低表达SDF-1α mRNA（P〈0．05）；经SDF-1α诱导后，P—PDLSCs中成血管相关基因CD31、VwFmRNA的表达水平均明显高于H—PDLSCs（P〈0．05）；其管腔形成数量也明显高于H-PDLSCs（P〈0．05）。结论：P—PDLSCs中CXCR4mRNA高表达，对牙周炎症组织环境中的SDF-1α更加敏感，其血管内皮向分化的趋势和能力均增强。

英文摘要：

AIM： To investigate the effects of SDF-1α/CXCR4 axis on the endothelial differentiation of pexiodontal ligament stem cells （PDLSCs）. METHODS： The stem cells from the periodontal ligament tissues of healthy subjects （H-PDLSCs） and periodontitis patients（P-PDLSCs） were isolated and cultured. Real time PCR were used to examine the expression of stromal cell-derived factor-1α（SDF-1α） and chemokine（ c-x-c motif）receptor4 （CXCR4） in H-PDLSCs and P-PDLSCs. The expression of endothelial gene CD31 and VWF in H-PDLSCs and P-PDLSCs after inclubation with SDF-1α was tested using real time PCR. H-PDLSCs and P-PDLSCs were induced by SDF-1α and seeded on matrigel, the capillary network and the tube formation were observed under phase contrast microscope. RESULTS： The expression of CXCR4 mRNA in P-PDLSCs was higher than in H-PDLSCs （P 〈0. 05 ）, but the expression of SDF-1α mRNA in P-PDLSCs was lower （P 〈 0.05）. After inclubation with SDF-1α the expression of CD31 and VWF in P-PDLSCs was higher than in H-PDLSCs （P 〈0.05）. Both H-PDLSCs and P-PDLSCs after inclubation with SDF-1α were able to create the capillary network and there were more tube formation in P-PDL-SCs than in H-PDLSCs（P 〈 0.05）. CONCLUSION： The expression of CXCR4 mRNA in P-PDLSCs is higher than in H-PDLSCs. P-PDLSCs are more sensitive to SDF-1α and have stronger capability of endothelial differentiation.