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中文摘要：
将DNA通过PDDA[poly（dimethyldiallylammonium chloride）]固定在单壁碳纳米管（CNT）表面，形成DNA—PDDA—CNT纳米复合体，用AFM、UV—Vis、Raman光谱及交流阻抗对其进行了表征．用伏安法研究了1-苯基偶氮-2-萘酚（PN）与固定在CNT表面的DNA的相互作用，结果表明PN与DNA的作用方式为嵌入作用；并且，PN能用作电化学检测DNA化学损伤的探针分子。本文电化学检测DNA损伤的优点在于PN的氧化还原式量电位E^0,≈0．1V（vs．SCE，pH5．5），能有效降低其它电活性物质对DNA损伤电化学检测的干扰。
英文摘要：
DNA-PDDA-CNT nanocomposites were fabricated by immobilizing DNA on the surface of single-walled carbon nanotubes （CNT） via poly（dimethyldiallylammonium chloride） （PDDA）. The atomic force microscopy （AFM）, ultraviolet-visible （UV-Vis）, Raman spectroscopy and impedance spectroscopy （EIS） were used to characterize the growth processes of the nanocomposites. The cyclic voltammetric results indicate that the interaction between 1-phenylazo-2-naphthol （PN） and the immobilized DNA is intercalative binding, and PN can be used as a probe for detection of DNA damage. PN seems to be an attractive probe for DNA damage analysis since its redox peak is at ca. 0.1 V （vs. SCE, pH 5.5）, where interference should be minimal.
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