中文摘要：

目的：研究不同浓度一氧化氮（NO）对肝癌细胞HepG2和外周血单个核细胞（PBMC）活性的影响。方法通过设置不同的PBMC、HepG2细胞密度及使用不同药物调节培养体系中NO的浓度，分别测量单独培养和间接共培养体系中两种细胞活性变化，并与不加药对照组进行统计分析。结果间接共培养的PBMC活性仅在NO浓度大于120μmol／L时受到影响，而与PBMC间接共培养的HepG2细胞在NO浓度小于对照组时和NO浓度大于241μmol／L时受到抑制。在不同的NO浓度条件下，间接共培养的HepG2细胞活性均高于单独培养的HepG2细胞。间接共培养体系中，随着PBMC与HepG2细胞数比值的升高，NO浓度降低，而HepG2细胞活性增加。结论培养液中过高或过低的NO浓度都对HepG2细胞有损害，过高浓度的NO对PBMC有损害，低浓度NO对PBMC无显著影响，间接接触PBMC对HepG2细胞的生长有促进作用。

英文摘要：

Objective To study the effects of different concentrations of nitric oxide （NO） on liver cancer cell line HepG2 and peripheral blood mononuclear cells （PBMC）. Methods Different rates of PBMC/HepG2 and drugs were arranged to regulate the concentrations of NO. Activity changes of the two kinds of cells were measured in cells cultured alone and co-cultured, which were compared with control group. Results The activity of PBMCs in co-cultured system was harmed only when the concentration of NO was more than 120 μmol/L, however, the activity of HepG2 cells in co-cultured system was suppressed when the concentration of NO was less than that of control group or was more than 241 μmol/L;moreover, the activity of co-cultured HepG2 cells was higher than that of HepG2 cells cultured alone in different concentrations of NO. In co-cultured system, with the increase of PBMC/HepG2 ratio, the concentration of NO was decreased, but the activity of HepG2 cells was increased. Conclusions The results have shown that HepG2 cells are damaged by high or low concentration of NO, and PBMC are only damaged by high concentration of NO, however, the low concentration of NO has few influences on PBMC. Moreover, the indirect contact of PBMC can promote the growth of the HepG2 cells.