中文摘要：

设计一种具有＂微坝＂和＂微缝＂结构的微流控芯片,能够物理隔离不同细胞,而且培养基中小分子营养物质可以自由流通。实验结果表明在芯片上可以共培养人肺腺癌细胞（A549）、人胚肺成纤维细胞（HLF-1）和人内皮细胞（HUVECs）三种细胞,在72 h培养后三种细胞生长状态良好,具有细胞图形化的特点和功能,为下一步开展多种细胞相互作用等相关研究提供重要的技术平台。

英文摘要：

Here a microfluidic chip with ‘micro-dam＇ and ‘micro-gap＇ has been designed and fabricated. It could isolate different cells and flow of medium in each region. It was found that the chip could realize the cells co-culture and patterning of human lung adenocarcinoma cell（A549）, human embryonic lung fibroblast（HLF-1） and human endothelial cells（HUVECs）, respectively. After 72 hours of culture, three kinds of cells grew well. It provided a developing technical platform for cell related research.