中文摘要：

当损害的一种批评类型由电离导致了放射，聚类的 DNA 损坏被考虑，它能在损坏在房间处理期间被变换成致命或强壮的诱变的复杂双海滨裂缝(DSB ) 。高精力质子生产的新数据表演更潜在地致命的 DSB 比低让的放射。在这研究， plasmid DNA 被用来调查并且重新估计生物效果在 vitro 在分子的水平与 3.6 keV/m 的 LET 一起由质子导致了，包括单个海滨裂缝(SSB ) ， DSB，孤立并且聚类的底损坏。复杂 DNA 损坏察觉的结果显示在给定的让的值的质子更导致大约 1.6 褶层 non-DSB 比迅速的 DSB 聚类 DNA 损坏。由质子的 DNA 损坏收益为聚类的损坏由为 DNA 损坏和 14 褶层的孤立的类型的 6 褶层明确地由 -rays, 比那大。而且，损坏的光谱也被表明被取决于放射质量，与比做光线相对簇生产更多的 DSB 的质子。

英文摘要：

Clustered DNA damage is considered as a critical type of lesions induced by ionizing radiation, which can be converted into the fatal or strong mutagenic complex double strand breaks （DSBs） during damage processing in the cells. The new data show that high energy protons produce more potentially lethal DSBs than low LET radiation. In this study, plasmid DNA were used to in- vestigate and re-evaluate the biological effects induced by the protons with the LET of -3.6 keV/μtm at the molecular level in vitro, including single strand breaks （SSBs）, DSBs, isolated and clustered base damages. The results of complex DNA damage detec- tions indicated that protons at the given LET value induce about 1.6 fold more non-DSB clustered DNA damages than the prompt DSB. The DNA damage yields by protons were greater than that by y-rays, specifically by 6 fold for the isolated type of DNA damage and 14 fold for the clustered damage. Furthermore, the spectrum of damages was also demonstrated to be depended on the radiation quality, with protons producing more DSBs relative to clusters than do γ-rays.