中文摘要：

比较7种大孔吸附树脂对革皮氏海参总皂苷的静态吸附和解吸效果,从中筛选出适合该海参总皂苷分离纯化的树脂,并对其动态吸附和解吸性能进行研究。结果表明,XAD761树脂最适合革皮氏海参总皂苷的纯化。当上样液质量浓度2mg/mL,流速0.8mL/min,上样液体积9BV时,树脂达到动态吸附饱和。再用5BV 70%乙醇,以1mL/min的流速可以洗脱完全,解吸率为93.31%。经过树脂的纯化,皂苷的纯度由原来的21%提高到65%。

英文摘要：

The adsorption and desorption rates of 7 types of macroporous resins towards total saponins from the sea cucumber Pearsonothria graeffei were investigated.Among the studied macroporous resins,XAD761 resin was the most suitable to purify crude total flavonoid extract from Pearsonothria graeffei.When 9 BV of 2 mg/mL sample was flowed through XAD761 resin column at a speed of 0.8 mL/min,the adsorption reached dynamic equilibrium.The adsorbed saponins could be completely eluted with 5 BV of 70%（V/V） ethanol at 1 mL/min,with a desorption rate of 93.31%.After the purification,the purity of total saponins was increased from 21% to 65%.