中文摘要：

草鲤鱼 reovirus (GCRV ) 是一试验在家庭 Reoviridae 的 Aquareovirus 类的成员。成熟 virion 包括由五核心蛋白质和二外部衣壳蛋白质组成的二同心 icosahedral 蛋白质壳围住的 11 个 dsRNA 染色体。染色体顺序和 3D 结构证明与家庭的另外的成员相比有在在 GCRV 和哺乳动物的 orthoreoviruses (MRV ) 之间的结构的蛋白质的顺序相同的高水平。理解 GCRV 感染的致病，外部衣壳蛋白质 VP5， MRV 的 μ1 蛋白质的相同，在 E.coli 被表示。recombinant VP5 高度被表示，这被发现，并且表示他的标签熔化蛋白质涉及包括身体的形成。另外，特定的 anti-VP5 浆液从净化的蛋白质被准备，西方的污点证明表示蛋白质能逻辑地把免疫绑在兔子反 GCRV 粒子浆液， immunogenicity 被 ELISA 试金决定。在调查 VP5 的功能的性质的另外的实验将进一步阐明在进在病毒的蛋白质和主人之中的主人房间，和它的相互作用的入口期间，房间在感染期间处理的 GCRV 外部衣壳蛋白质 VP5 的角色。

英文摘要：

Grass carp reovirus （GCRV） is a tentative member of the Aquareovirus genus in the family Reoviridae. The mature virion comprises 11 dsRNA genomes enclosed by two concentric icosahedral proteins shells that is comprised of five core proteins and two outer capsid proteins. The genome sequence and 3D structure demonstrate there is a higher level of sequence homology in structural proteins between GCRV and mammalian orthoreoviruses （MRV） compared to other members of the family. To understand the pathogenesis of GCRV infection, the outer capsid protein VP5, a homology of the μ1 protein of MRV, was expressed in E.coli. It was found that the recombinant VP5 was highly expressed, and the expressed His-tag fusion protein was involved in the formation of the inclusion body. Additionally, specific anti-VP5 serum was prepared from purified protein and western blot demonstrated that the expressed protein was able to bind immunologically to rabbit anti GCRV particle serum and the immunogenicity was determined by ELISA assay. Additional experiments in investigating the functional properties of VP5 will further elucidate the role of the GCRV outer capsid protein VP5 during entry into host cells, and its interaction among viral proteins and host cells during the infection process.