中文摘要：

目的：筛选能够转化白头翁皂苷的真菌，并对该菌株进行分类鉴定。方法：将白头翁皂苷加入培养基中诱导真菌分泌白头翁皂苷糖苷酶，并通过薄层层析技术检测转化产物。再采用经典的形态学特征观察和现代的ITS—rDNA测序分子生物学分类技术相结合对真菌进行鉴定。结果：筛选出能够生物转化白头翁皂苷的RCEF4894菌株，该菌株被鉴定为棘孢曲霉Aspergillus aculeatus Iizuka。结论：棘孢曲霉RCEFd894菌株能够被诱导产生白头翁皂苷糖苷酶，将多糖基的白头翁皂苷水解成低糖基的皂苷。

英文摘要：

Objective： The fungi which could biotransform pulchinenoside were selected, and they were classified and identified. Methods： The pulchinenoside was supplemented into the culture medium to induce secretion of pulchinenoside - glycosidase by the fungi, and the pulchinenoside was detected by silica gel thin - layer chroma- tography. The fungi was identified based on its ITS -rDNA sequence and the morphological characteristics. Resuits： The RCEF4894 strain biotransformed pulchinenoside was isolated and identified as Aspergillus aculeatus Iizuka. Conclusion： The RCEF4894 strain could be induced to produce the pulchinenoside -glycosidase, which hydrolyzed the polyglycosylation of pulchinenoside into underglycosylation of pulchinenoside.