中文摘要：

目的：观察辛伐他汀对骨髓源性平滑肌祖细胞（SPC ）分化及增殖的影响，筛选新一代包被洗脱支架药物。方法：采用密度梯度离心法从大鼠骨髓获取单个核细胞，接种于纤维连接素包被的培养板，培养8d后，以平滑肌肌动蛋白（α-SM A ）免疫荧光染色鉴定骨髓源性SPC ，并在倒置荧光显微镜下计数。收集培养8d单个核细胞、贴壁细胞，分别加入不同浓度辛伐他汀（0.01-10μmol/L ）培养8d、24h。分别采用氚标记胸苷（3 H-TdR）摄入法、改良的Boyden小室和黏附能力测定实验观察SPC的分化、增殖、迁移和黏附能力。结果：与对照组（无辛伐他汀干预）相比，0.01μmol/L辛伐他汀显著抑制骨髓单个核细胞向SPC分化[（85±4）个比（79±5）个]、增殖[（4070±184）个比（3833±126）个]、迁移[（44±3）个比（39±3）个]和黏附[（59±5）个比（52±4）个]， P均＜0.05，且随辛伐他汀浓度增加S PC数量显著减少（ P＜0.01）。结论：辛伐他汀可抑制骨髓源性平滑肌祖细胞分化、增殖、迁移及黏附能力。

英文摘要：

Objective：To observe influence of simvastatin on differentiation ,proliferation ,migration and adhesion of marrow-derived smooth muscle progenitor cells （SPCs） and screen coated eluting stent drugs of new generation . Methods ：The mononuclear cells （MNCs） were isolated from rat marrow by density gradient centrifugation method , and then plated on fibronectin-coated culture dishes ,after culture 8d ,marrow-derived SPCs were identified by α-smooth muscle actin （α-SMA） immunofluorescent staining and counted under inverted fluorescence microscope .The MNCs and adhesion cells were treated with simvastatin （0.01-10 μmol/L） respectively for 8 d and 24h .SPCs pro-liferation ,migration and adhesion were observed by Tritium thymidine （3 H-TdR） intake method ,modified Boyden chamber assay and adhesion assay .Results：Compared with control group （no simvastatin intervention ） ,0.01μmol/L simvastatin significantly inhibited the MNCs differentiation towards SPCs [ （85 ± 4） vs .（79 ± 5）] ,proliferation [ （4070 ± 184） vs .（3833 ± 126）] ,migration [ （44 ± 3） vs .（39 ± 3）] and adhesion of SPCs [ （59 ± 5） vs .（52 ± 4）] , P〈0.05 all ,and number of SPCs significantly reduced along with simvastatin concentration increased （P〈0.01） . Conclusion：Simvastatin could inhibit the differentiation ,proliferation ,migration and adhesion of marrow-derived smooth muscle progenitor cells .