中文摘要：

通过对小花棘豆内生真菌的分离纯化,筛选产苦马豆素内生真菌,探讨小花棘豆内生真菌与其毒性成分苦马豆素的相互关系。本研究对采自内蒙古西部草地的6份小花棘豆样品进行内生真菌分离纯化,采用薄层色谱和气相色谱技术对内生真菌发酵液进行苦马豆素定性、定量检测,筛选产苦马豆素内生真菌;结合形态学和分子生物学方法进行内生真菌种属鉴定。从小花棘豆样品中分离得到6株内生真菌。经筛选,其中有2株产苦马豆素,编号为XJ-J-I和XJ-H-1,其发酵液中苦马豆素产量分别为4.209 2mg.L-1和16.733 8mg.L-1。鉴定结果表明,这2株内生真菌虽然形态学存在差异,但基因型相同,同属镰刀属内生真菌—层出镰刀菌（Fusarium prolifera-tum）。小花棘豆中存在产苦马豆素内生真菌。

英文摘要：

To screen and obtain swainsonsine-producing endophytic fungi in Oxytropis.flabra,explore the relationship between endophytic fungi and swainsonine toxin in Oxytropis.flabra.In this research,we isolated and purified endophytic fungi of 6 Oxytropis.flabra samples from the west Inner Mongolia.Swainsonine was detected by thin layer chromatography and gas chromatography in fermentation liquor of the isolated fungus,then screened the Swainsonsine-producing endophytic fungi.Combined colonial morphology and micro-morphology to identify the species of isolated fungus.Finally got 6 species of endophytic fungi through identification.2 of the 6 species endophytic fungi can produce swainsonine,named them XJ-J-1 and XJ-H-1 respectively.The Swainsonine yield was determined by gas chromatography,4.209 2 mg·L-1 in the fermentation liquor of XJ-J-1 and 16.733 8 mg·L-1 in the fermentation liquor of XJ-H-1.The 2 strains endophytic fungi have the same genotype but different phenotype,both of them are Fusarium proliferatum.The results show that the swainsonine-producing endophytic fungi can be isolated from the Oxytropis.flabra.