中文摘要：

目的：探讨IL-8作用下人肺腺癌A549细胞的迁移率变化以及线粒体融合与分裂基因的变化。方法：实验分为对照组和IL-8作用组。采用细胞划痕实验方法观察IL-8作用下细胞迁移能力的变化;Transwell小室检测细胞的迁移率;ELISA实验检测不同迁移率的细胞内源性IL-8的分泌量;Western blot法检测线粒体外膜蛋白Tom20及线粒体膜间腔蛋白细胞色素C（cytochrome C,Cyt C）的表达。RT-PCR检测线粒体融合基因Mfn1、Mfn2、OPA1及分裂基因Fis1、Drp1、MTP18的表达;Mito Tracker Red CMXRos染色激光共聚焦显微镜观察细胞中线粒体的形态。结果：肺腺癌A549细胞的迁移率高于SPC-A-1细胞,且内源性IL-8的分泌也高于SPC-A-1细胞。IL-8作用下A549细胞的迁移率增加,并存在时间依赖性。与对照组比较,IL-8作用组的A549细胞线粒体膜间腔蛋白Cyt C蛋白表达降低（P〈0.05）,外膜蛋白Tom20表达增加（P〈0.05）,线粒体外膜融合基因Mfn1及Mfn2表达增加（P〈0.05）,内膜融合基因OPA1表达降低（P〈0.05）,分裂基因Fis1无明显变化（P〉0.05）,Drp1及MTP18增加（P〈0.05）;激光共聚焦显微镜显示IL-8作用下线粒体形态发生变化,出现点状聚集。结论：A549细胞在IL-8作用下迁移率增加,可能与A549细胞线粒体融合与分裂基因变化有关。

英文摘要：

AIM：To investigate the changes of migration of lung adenocarcinoma cells promoted by IL-8 and the inner and outer mitochondrial membrane dynamic changes during this process.METHODS：Human lung adenocarci-noma cell line A549 was divided into control group and IL-8 group.Cell migration was analyzed by scratch detection and Transwell assay.The secretion of endogenous IL-8 was detected by ELISA.The protein levels of mitochondrial cytochrome C （ Cyt C） and mitochondrial outer membrane protein Tom20 was detected by Western blot.The mRNA expression of mito-chondrial fusion genes Mfn1, Mfn2 and OPA1 and fission genes Fis1, Drp1 and MTP18 was detected by RT-PCR.The morphological changes of mitochondria were observed by MitoTracker Red CMXRos dye staining and confocal microscopy. RESULTS：The migratory rate of A549 cells and endogenous secretion of IL-8 in A549 cells were higher than those in SPC-A-1 cells.The migratory rate of A549 cells was improved by IL-8 in a time-dependent manner.Compared with control group, the Tom20 protein expression was increased （ P〈0.05 ） , and the Cyt C protein expression was decreased （ P〈0.05）.The expression of mitochondrial outer membrane fusion genes Mfn1 and Mfn2 was increased （P〈0.05）, and the expression of mitochondrial inner membrane fusion gene OPA1 was decreased （P〈0.05）.The expression of fission genes Drp1 and MTP18 were decreased （P〈0.05）, while the expression of Fis1 was no change （P〉0.05）.Under confocal microscope, the punctate aggregates in the mitochondria of the A549 cells treated with IL-8 were observed.CONCLU-SION：The migratory rate of A549 cells is increased by IL-8, which is related to the changes of mitochondrial fusion genes and the fission genes.