中文摘要：

目的：探讨抗凋亡蛋白Bcl-2、基质金属蛋白酶-2（MMP-2）和MMP-9在肝细胞肝癌中表达的关联性，及其与复发、转移和预后的关系。方法：以石蜡包埋组织切片，免疫组织化学SP法染色检测97例肝细胞肝癌，分别在复发与转移组49例和非复发与转移组48例中分析Bcl-2核表达和MMP-2、MMP-9表达的情况。结果：复发与转移组Bcl-2核表达高于无复发与转移组（x。=7．912，P=0．005），复发转移组MMP一9表达高于无复发转移组（x^2=6．545，P=0.011），Bcl-2核表达阳性细胞率与MMP一9阳性细胞率存在相关性，而与MMP-2无关。Bcl-2核表达组患者生存时间较短，差异具有统计学意义。结论：Bcl-2核表达阳性患者更易出现复发与转移，且与MMP-9过度表达存在关联性，表明Bcl-2入核可能对肿瘤转移的相关生物学功能具有调节作用，可作为评价HCC复发与转移、不良生存预后的候选临床标志。

英文摘要：

Objective： To determine the correlation of the expression of the anti-apoptotic protein Bcl-2 with the expression of matrix metalloproteinases MMP-2 and MMP-9 in human hepatocellular carcinoma, and their relationship with recurrence, metastasis, and prognosis. Methods： Paraffin-embedded tissue sections of 97 hepatocellular carcinoma cases were subjected to SP immunohisto- chemical staining. The nuclear expression of Bcl-2 as well as the expression of MMP-2 and MMP-9 were detected. A total of 49 cases had recurrence and metastasis, and the remaining 48 did not. Results： The nuclear expression of Bcl-2 was higher in cases with recur- rence and/or metastasis （ t = -2.488, P = 0.015 ）. Similarly, MMP-2 and MMP-9 expression were higher in cases with recurrence and/or metastasis （ Z =-2.623, P = 0.009; Z =-2.028, P = 0.043 ）. The nuclear expression of Bcl-2 was correlated with the expression of MMP-9, but not with that of MMP-2. Patients with positive nuclear expression of Bcl-2 had shorter survival time, and the difference was significant. Conclusion： The nuclear expression of Bcl-2 was significantly correlated with metastasis and MMP-9 expression. Bcl-2 relocation into the nucleus may regulate metastasis-related proteins, which can be used as a clinical marker for evaluating recurrence, metastasis, and prognosis.