中文摘要：

客观 Interleukin-1 尾(IL-1 尾) 在类型 1 和类型 2 糖尿病 mellitus 的发展起一个重要作用。Resveratrol，多酚，被知道在 vitro 有大量药理学性质。在这研究，我们在 IL-1 尾 - 上检验了 resveratrol 的效果导致的尾 - 房间机能障碍。我们首先在氮的氧化物上评估了 resveratrol 的效果的方法(没有) 在用 Griess 方法与 IL-1 尾刺激的 RINm5F 房间的形成。下次，我们执行了短暂 transfection 和记者试金测量 peroxisome 的 transcriptional 活动激活 proliferator 的受体 -- 纬(PPAR- 纬) 。我们也使用了西方的弄污的分析在可诱导的氮的氧化物 synthase (iNOS ) 上估计 resveratrol 的效果表示和原子因素 -- 到在房间的原子核的 translocation 与 IL-1 尾对待的魏 B (NF-魏 B ) 。另外，我们用 electrophoretic 活动性移动试金(EMSA ) 估计了 NF-魏 B 的 transcriptional 活动。最后，我们在刚孤立的老鼠在刺激葡萄糖的胰岛素分泌物的 IL-1 尾鈥搃n duced 抑制上评估了 resveratrol 的效果胰腺的小岛。结果 Resveratrol 显著地压制了导致的 IL-1 尾 - 没有生产，一发现那与 iNOS mRNA 和蛋白质的减少的层次相关很好。resveratrol 由禁止了 iNOS 基因表示的分子的机制看起来包含增加的 PPAR- 纬活动，它导致了 NF-魏 B 激活的抑制。进一步的分析证明 resveratrol 能阻止 IL-1 尾 - 在老鼠小岛的刺激葡萄糖的胰岛素分泌物的导致的抑制。在这研究的结论，我们证明 resveratrol 能免于房间机能障碍由 IL-1 尾引起了的胰腺的尾 - 。

英文摘要：

Objective：Interleukin-1β（IL-1β）plays an important role in the development of type 1 and type 2 diabetes mellitus.Resveratrol,a polyphenol,is known to have a wide range of pharmacological properties in vitro.In this research,we examined the effects of resveratrol on IL-1β-inducedβ-cell dysfunction.Methods：We first evaluated the effect of resveratrol on nitric oxide（NO）formation in RINm5F cells stimulated with IL-1βusing the Griess method.Next,we performed transient transfection and reporter assays to measure the transcriptional activity of peroxisome proliferator-activated receptor-γ（PPAR-γ）.We also used Western blotting analysis to assess the effect of resveratrol on inducible nitric oxide synthase（iNOS）expression and nuclear factor-κB（NF-κB）translocation to the nuclei in cells treated with IL-1β.In addition,we assessed the transcriptional activity of NF-κB using an electrophoretic mobility shift assay（EMSA）.Finally,we evaluated the effect of resveratrol on IL-1β-induced inhibition of glucose-stimulated insulin secretion in freshly isolated rat pancreatic islets.Results：Resveratrol significantly suppressed IL-1β-induced NO production,a finding that correlated well with reduced levels of iNOS mRNA and protein.The molecular mechanism by which resveratrol inhibited iNOS gene expression appeared to involve increased PPAR-γactivity,which resulted in the inhibition of NF-κB activation.Further analysis showed that resveratrol could prevent IL-1β-induced inhibition of glucose-stimulated insulin secretion in rat islets.Conclusion：In this study,we demonstrated that resveratrol could protect against pancreaticβ-cell dysfunction caused by IL-1β.