中文摘要：

目的：探讨运动对大鼠海马长时程增强（long-term potentiation,LTP）效应及其相关因子的影响。方法：28只成年雄性Wistar大鼠,随机分为对照组与运动组,每组14只。运动组采用4周自愿跑轮运动为体力运动模型。脑立体定位-神经电生理法检测海马齿状回LTP;高效液相-电化学法测定海马5-羟色胺（5-HT）含量;实时荧光定量PCR测定海马5-HT1A受体、环磷酸腺苷反应元件结合蛋白（CREB）和脑源性神经营养因子（BDNF）mRNA表达。结果：运动组海马齿状回LTP较对照组明显提高（P〈0.05）,运动组海马5-HT含量（P〈0.01）、5-HT1A受体mRNA（P〈0.01）、CREB和BDNF mRNA表达水平也显著高于对照组（P〈0.05）。结论：运动可能通过对5-HT—5-HT1A受体—cAMP—CREB—BDNF—LTP通路元件的上调机制,发挥增强海马LTP的作用。

英文摘要：

Objective To study the mechanisms underlying physical exercise produced long-term potentiation （LTP） enhancement in hippocampus of rats. Methods Twenty Eight adult male Wistar rats were randomly divided into control group （sedentary group） and exercise group （4-week voluntary wheel running）. LTP in hippocampal dentate gyrus （DG） was recorded by stereotaxic neuronal electrophysiological methods. Levels of hippocampal 5-HT were detected by high-performance liquid chromatography using electrochemical detection. The mRNAs for 5-HT1A receptor, cAMP-response-element binding protein （CREB） and brain-derived neurotrophic factor （BDNF） were measured by real-time fluorescence quantitative reverse transcription polymerase reaction （RT-PCR）. Results Compared with the sedentary controls, enhanced hippocampal LTP （P（0. 05）, increased hippocampal 5-HT levels （P〈0. 01）, up-expressed mRNA levels for 5-HT1A receptor （P〈0. 01）, CREB and BDNF （P〈0. 05） in rats were found in 4 weeks physical exercise group. Conclusion 5-HT, 5-HT1A receptor, CREB and BDNF were likely act as important modulators to be involved in exercise-induced LTP promotion process, in which up-regula- tion of the related factors in 5-HT--5-HT1A receptor--cAMP--CREB--BDNF-LTP pathway might take action.