中文摘要：

为了深入了解蛋白质-核酸相互作用模式,对复合物结构中氢键/范德华力作用力位点上的氨基酸和核苷酸的偏好性（即相对使用频率）进行了统计分析.结果表明：氢键/范德华力作用位点上对氨基酸的偏好性差异均极其显著,与蛋白质-核酸特异作用密切相关的残基侧链与核苷酸碱基间相互作用力位点对氨基酸类型的选择特异性更高;单链RNA分子与蛋白质发生相互作用时对氨基酸残基的偏好性和双链RNA分子差异不显著;氨基酸的极性大小及方向在决定其是否与核酸分子形成范德华力作用具有重要贡献,氨基酸侧链形成的空间位阻会阻碍其与核酸分子形成氢键作用;蛋白质-DNA复合物结构氢键/范德华力作用位点上残基间空间协同作用时对氨基酸类型的选择与蛋白质-RNA复合物结构间存在显著差异.

英文摘要：

To further explore the motifs of protein-nucleic acid interaction,the propensities（i.e.relative frequency）of amino acids and nucleotides usage at protein-nucleic acid interfaces by hydrogen bonds and van del Waals contacts are analyzed.The results show that it presents significant differences among propensities of amino acids recognizing nucleotides by hydrogen bonds or van der Waals contacts in protein-nucleic acid complex structures,in addition,specific interacting sites between atoms of the side chain of amino acids and base atoms present higher propensity for choosing amino acids;propensities of amino acids at hydrogen bonding/van del Waals contacting interfaces among the interaction of protein with single-stranded RNA molecules present no significant differences with those among the interaction of protein with double-stranded RNA molecules;the size and orientation of the polarity of amino acids play important roles in determining whether they act with nucleic acid molecules by van der Waals contacts,and the steric hindrances formed by the side chain of amino acids appear to influence the course of formation of hydrogen bonds between amino acids and nucleotides;the choice of the type of amino acids for space-adjacent residues with cooperative interactions at hydrogen bonding/van del Waals contacting interfaces of protein-DNA complexes are significantly different from those of protein-RNA complexes.