中文摘要：

目的：利用生物信息学方法对变应性鼻炎并发哮喘机制进行初步探讨。方法：利用GenMAPP软件对季节性变应性鼻炎（SAR）与SAR并发哮喘患者鼻黏膜组织Affymetrix寡核苷酸芯片表达谱结果进行分析。首先筛查差异表达基因,然后对差异基因做数据库检索,并对其生物路径进行分析。结果：在38 500多个基因中（47 000个转录本中）,SAR并发哮喘者,其鼻黏膜发生4倍以上差异表达的基因共有689个,其中有233个基因表达上调,456个基因表达下调。MAPPfindder分析示这些差异表达基因参与了69条生物路径。其中细胞因子与细胞因子受体间作用路径中差异表达基因最多,且在此路径中趋化因子CXCL12及其受体CXCR4在变应性鼻炎并发哮喘患者中较单纯变应性鼻炎患者表达均发生显著上调。结论：变应性鼻炎及其并发哮喘过程中许多生物路径参与此过程,其中CXCL12/CXCR4相互作用构成的反应轴在气道变应性疾病中发挥着重要作用。

英文摘要：

Objective：To detected the mechanism of allergic rhinitis associated with asthma with bioinformatics methods.Method：GenMAPP software was used to analyze the expression profile of nasal mucosa of seasonal allergic rhinitis（SAR） and SAR associated with asthma of oligonucleotide microarray（Affymetrix HG-U133-plus2）.One the first step,of differentially expressed genes screening were done,then differential gene database retrieval was established,at last pathway analysis was performed.Result：689 genes out of 47 000 analyzed transcripts of nasal mucosa of SAR associated with asthma were differentially expressed at least 4-fold,in which 233 genes were up regulated and 456 genes were down regulated.These differential expression genes participate in 69 bio-pathways,in which the interaction pathway between cytokine and cytokine receptor was most.Chemotatic factor CXCL12 and its receptor CXCR4 expressed in SAR associated with asthma patients were up-regulated predominantly,compared with that in SAR patients.Conclusion：Mutiple pathways were involved in the development of SAR and SAR complicated with asthma.The CXCL12/CXCR4 axis might play a main role in the allergic airway diseases.