中文摘要：

目的 探讨血清胸苷激酶1（TK1）检测作为肿瘤细胞增殖标志物对肿瘤早期预测的意义,探讨血清TK1的含量与肿瘤病种、复发转移、临床分期及临床治疗效果评估的关系。方法 应用酶免疫点印迹化学发光法测定144例肿瘤患者（肿瘤患者组）和67例健康对照者（对照组）血清TK1的水平,分析血清TK1与肿瘤患者各生物学行为参数的关系。结果 肿瘤患者组和对照组在血清TK1水平及阳性表达率间的差异均有统计学意义（P〈0.01）,且TK1浓度与年龄呈正相关性（P〈0.01）。肿瘤患者TK1的表达在不同病种之间的差异有统计学意义（P〈0.05）,有复发或转移的患者血清TK1阳性表达率与无复发或转移的患者比较差异有统计学意义（P〈0.01）,大肠癌及肺癌的Ⅰ期、Ⅱ期、Ⅲ期、Ⅳ期,各组血清TK1阳性表达率差异有统计学意义（P〈0.01）。大肠癌患者治疗后不同临床疗效（完全缓解,部分缓解,疾病进展）的患者血清TK1水平的差异有统计学意义（P〈0.01）。结论 TK1可用于对恶性肿瘤的早期筛查。血清TK1检测可作为临床上对肿瘤增殖程度及复发监测的手段之一。

英文摘要：

Objective To evaluate the use of serum thymidine kinase 1 （ TK1 ） as a proliferating tumor marker for early tumor detection. To investigate possible correlations between TK1 levels and categories, recrudescent or me-tastasizes , clinical staging levels and curative effect. Methods Using enhanced chemo-luminescence assay to de-tect the serum TK1 levels of 144 cancer patients and 67 healthy controls. Analyzing the relationship between the bi-ological behavior of cancer parameters and the TK1 levels. Results The concentration of TK1 in cancer patients was significantly higher compared to the control group （ P〈0. 01 ） . There exists significant difference in both TK1 concentrations and expression between cancer patients and the control group （ P〈0. 01 ） . The positive rate of TK1 expression was found to increase as age increased（P〈0. 01）. There exists significant difference in TK1 expression between different categories （ P〈0. 05 ） . There was also significant difference in TK1 expression between patients with recrudescent or metastasizes and patients without recrudescent or metastasizes （ P 〈0. 01 ） . The significant difference in TK1 expression was found between different clinical staging （Ⅰ,Ⅱ,Ⅲ,Ⅳ） of colorectal and lung cancers （ P〈0. 01 ） . There exists significant difference in TK1 concentration between untreated and post-treated cancer patients. The significant difference of concentrations of TK1 was found in routine clinical setting therapy effects （CR/PR/PD） in post-treated cancer patients （P〈0. 01）. Conclusion TK1 can predict the risk for devel-opment of neoplasia related diseases in its early stages. Concentration levels of TK1 can monitor tumor growth as well as predict the likelihood of recurrence in cancer patients.