中文摘要：

基于纳米纤维的富集作用,建立了血浆中5-羟色胺（5-HT）的柱前衍生高效液相色谱-电化学检测（HPLC-ECD）分析方法。用10%（v/v）高氯酸溶液沉淀血浆蛋白,离心后取上清液,用0.1 mo l/L的四苯硼酸钠溶液调节pH值至8.5,加入衍生剂邻苯二甲醛溶液于30℃衍生4 min,经纳米纤维固相萃取柱净化富集后,以pH 5.4的0.05 mo l/L磷酸二氢钾缓冲液（含0.25 mmo l/L乙二胺四乙酸）-甲醇（60∶40,v/v）为流动相在C18色谱柱（150mm×4.6 mm,5μm）上进行分离,进样量为20μL。结果表明,在高、中、低3个加标水平下,血浆中5-HT的加标回收率为95.6%～101.4%,日内与日间相对标准偏差均小于5%（n=3）。方法的定量线性范围为5～500μg/L,检出限为1μg/L。该方法操作简单、快速,具有较高的灵敏度和较高的重现性,能够用于血浆中5-HT的含量测定。

英文摘要：

A novel packed-nanofiber solid phase extraction coupled with high performance liquid chromatography-electrochemical detection（HPLC-ECD） method was established for the determination of 5-hydroxytryptamine（5-HT） in plasma.A 10%（v/v） HClO4 solution was used to precipitate the protein in plasma samples.After homogenizing for 1 min and centrifuging for 10 min at 12 000 r/min,the supernatant was adjusted to pH 8.5 with 0.1 mol/L sodium tetraphenylborate and then derivatized with o-phthalaldehyde（OPA） solution at 30 ℃ for 4 min.The solution was then purified and preconcentrated by the packed-nanofiber solid phase extraction column,in which methanol was used as the eluent.The analyte was analyzed by HPLC-ECD.The chromatographic separation was achieved on a Shimadzu C18 column with pH 5.4 0.05 mol/L phosphate buffer solution（containing 0.25 mmol/L ethylene diamine tetraacetic acid）-methanol（60∶40,v/v） as mobile phase.The linear range was 5-500 μg/L with the correlation coefficient（r2） of 0.999 6.The limit of detection（S/N=3） was 1 μg/L.And the spiked recoveries were in the range of 95.6%-101.4% with the relative standard deviations（RSDs） of intra-day and inter-day assays below 5%（n=3）.On the basis of the advantages of simplicity,high sensitivity and good reproducibility,this method can be used for the determination of 5-HT in human plasma.