中文摘要：

目的研究不同脂肪酸构成对小鼠血脂代谢、炎症和氧化应激及内皮细胞功能的影响。方法雄性KM小鼠随机分为5组,分别喂饲正常对照饲料、猪油高脂饲料和n-6/n-3多不饱和脂肪酸（PUFA）比值为1∶1、5∶1、20∶1的高脂饲料5周,比较各组小鼠血清中甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、白介素-6（IL-6）、丙二醛（MDA）、超敏C反应蛋白（hsCRP）、肿瘤坏死因子（TNF-α）、脂质过氧化物（LPO）、8-异前列腺素F2α（8-isoPGF2α）、氧化型低密度脂蛋白（ox-LDL）、游离脂肪酸（FFA）、选择素（ES）和血管性血友病因子（v WF）的含量变化。结果猪油组LDL-C和非高密度脂蛋白胆固醇（non-HDL-C）水平显著高于其他各组（P〈0.05）。n-6/n-3 PUFA 1∶1组和5∶1组血清TG、TC水平显著低于猪油组（P〈0.05）。20∶1组血清FFA水平显著高于1∶1组和5∶1组（P〈0.05）。1∶1组和5∶1组血清炎症因子和氧化应激指标及ES水平均显著低于猪油组和20∶1组（P〈0.05）。5∶1组血清v WF水平显著低于猪油组和20∶1组（P〈0.05）。结论与猪油和高n-6/n-3 PUFA比值高脂饲料相比较,低n-6/n-3PUFA比值高脂饲料可改善小鼠脂代谢、炎症与氧化应激和内皮细胞功能。

英文摘要：

Objective To investigate effects of diets with different fatty acid composition on serum lipid profiles, inflammation, oxidative stress and endothelial function in mice fed high-fat diets. Methods Male KM mice were randomly divided into five groups and were fed normal control diet,high-fat lard diet,high-fat diets with n-6 / n-3 polyunsaturated fatty acid（ PUFA） ratios of 1 ∶ 1,5 ∶ 1 and 20 ∶ 1 for five weeks,respectively. The levels of serum triglyceride（ TG）,total cholesterol（ TC）,high-densitylipoprotein cholesterol（ HDL-C）, low-density lipoprotein cholesterol（ LDL-C）,interleukin-6（ IL-6）,malondialdehyde（ MDA）,hypersensitive C-reactive protein（ hsCRP）,tumor necrosis factor alpha（ TNF-α）,lipid peroxide（ LPO）,8-iso prostaglandin F2α（ 8-iso-PGF2α）,oxidized low-density lipoprotein（ ox-LDL）,free fatty acid（ FFA）,E-selectin（ ES） and von Willebrand factor（ v WF） were measured. Results The levels of serum LDL-C and non-HDL-C in the lard group were significantly higher than those in the other groups（ P 0. 05）. The lard group had significantly higher serum TG and TC concentrations compared to 1∶ 1 and 5∶ 1 groups（ P〈0. 05）. The levels of serum FFA in20∶ 1 group were significantly higher than those in 1 ∶ 1 and 5 ∶ 1 groups（ P〈0. 05）.Compared with the lard and 20∶ 1 groups,the 1∶ 1 and 5∶ 1 groups displayed lower levels of inflammatory cytokines,oxidative stress and ES. The 5∶ 1 group significantly decreased the level of serum v WF compared to lard and 20 ∶ 1 groups（ P〈0. 05）. Conclusion High-fat diet with low n-6 / n-3 PUFA ratio could improve lipid metabolism,inflammation,oxidative stress and endothelial function compared to high-fat diets with lard and higher n-6 / n-3 PUFA ratio. The diet with low n-6 / n-3 PUFA ratio can improve cardiovascular disease risk factors to prevent cardiovascular disease.