中文摘要：

目的 观察小鼠骨关节炎模型中关节软骨组织中基质金属蛋白酶13（matrix metalloproteinase 13，MMP-13）表达水平的变化，探讨其与关节损伤之间的关系。 方法 在小鼠原代软骨细胞培养中用IL-1β诱导炎症细胞外环境，采用RT-PCR检测MMP-13的表达水平。手术诱导不稳定的内侧半月板（destabilization of the medial meniscus，DMM）建立小鼠骨关节炎模型，观察术后5周和10周小鼠关节软骨组织变化情况，并用免疫组化检测MMP-13在受损的关节软骨中的表达。 结果 用IL-1β处理的原代软骨细胞中MMP-13的mRNA表达明显较高（P〈0.01）。DMM术后小鼠关节软骨出现骨关节炎表现，且术后10周小鼠关节损伤程度较术后5周小鼠严重。免疫组化结果显示术后5周小鼠软骨组织中MMP-13的表达高于对照组；术后10周软骨组织中MMP-13进一步增高。 结论 在小鼠骨关节炎模型发病过程中，MMP-13作为一个具有软骨破坏性的标志分子在关节软骨中持续高表达，在骨关节炎发生过程中起重要作用。

英文摘要：

Objective To determine the change of matrix metalloproteinases 13 （MMP-13） expression in the articular cartilage of osteoarthritis mice and investigate its correlation with articular injury. Methods Murine articular chondrocytes were isolated from neonatal mice and cultured primarily, and then treated with 10 ng/mL interleukin-1β （IL-1β） for 24 h. The mRNA expression of MMP-13 was detected by quantitative real-time reverse transcriptase-polymerase chain reaction. A total of 30 C57BL/6J mice were randomly divided into 3 groups, normal control （n=10）, osteoarthritis group 1 （n=10） and osteoarthritis group 2 （n=10）. The osteoarthritis model was induced by surgical destabilization of the medial meniscus （DMM）. Ten mice of the model group 1 were killed in 5 weeks after surgery, while the other 10 of group 2 were in 10 weeks. The articular cartilage was observed histologically, and the expression level of MMP-13 in impaired articular cartilage was measured by immunohistochemical assay. Results IL-1β induced increased mRNA expression of MMP-13 in primary cultured murine articular chondrocytes. Articular cartilage in mice with DMM surgery exhibited features of osteoarthritis, and the injury became more severe with the time elapsing. Immunohistochemical assay showed that the expression of MMP-13 was enhanced in 5 weeks after DMM surgery, and continually increased in 10 weeks post-operatively. Conclusion MMP-13, as a chondro-destructive marker, is of high expression in the articular cartilage, and plays an essential role in the process of osteoarthritis.