中文摘要：

通过改良的CTAB方法提取桃叶片总RNA,根据桃PGIP基因开放阅读框设计特异引物,以荧光实时定量PCR技术分析低浓度SA诱导处理后桃叶片PGIP基因的表达水平变化。结果表明：0.002mmol/L SA诱导处理桃叶片后引起PGIP基因表达水平上升,在2h出现峰值,表达峰值时（2h）的表达量是最低点（8h）表达量的2.4倍。清水对照在0～8h内PGIP基因的表达几乎没有变化,由此可见,0.002mmol/L的SA对桃叶片PGIP基因的表达有促进作用。

英文摘要：

In this research,the total RNA of peach leaves were extracted with the improved CTAB method.Specific oligonucleotide primers were designed according to the peach PGIP gene＇s open reading frame.According to the method of real-time fluorescence quantitative PCR,the expression changing of peach leaves and peel were analyzed.The result showed that 0.002 mmol/L SA treatment resulted in up-regulation of PGIP gene expression.The expression peak occurred 2 h after treatment.The highest level（2 h） was 2.4 times higher than the lowest level（8 h）.The expression of PGIP gene of the control samples were nearly unchanged in 0~8 h,which showed that 0.002 mmol/L SA could promote the expression of PGIP gene.