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中文摘要：
从湖北省某皂素生产废水池底污泥中分离出2株耐高盐（CaCl2）皂素废水降解菌，编号分别为B723—1和B723—3，并进行菌株的鉴定和生长特性以及不同盐度迫胁下胞内游离氨基酸、蛋白质、膜的相对透性的研究，结果发现：（1）菌株B723—1与扩展短杆菌的同源性为98．0％～99．0％，B723—3与松鼠葡萄球菌的同源性为98．0％～99．0％：（2）两菌株均能在0．0％～9．0％CaCl2范围内生长良好；（3）菌株B723—1和B723—3的适宜生长温度分别是20～35oC和25～37oC，两者均在pH7．0～10．0生长；（4）当耐盐菌株受到1．8％、3．6％、5．4％和9．0％CaCl2盐迫45min时，其胞内会迅速积累大量氨基酸来平衡外界的高盐渗透压，且其胞内蛋白质含量均高于对照组（未受盐迫），而其膜的相对通透性低于非耐盐菌株．图4表1参11.
英文摘要：
B723-1 and B723-3, two tolerant-CaCl2 and diosgenin degrading strains were isolated from the sludge in diosgenin wastewater pond in Hubei Province. Their growth characteristics, contents of free amino acids, protein and membrane permeability under different CaCl2 stresses were studied. The result indicated ： （ 1 ） strain B723-1 was closely related to Brevibacterium linens by 98.0% - 99.0% similarity and strain B723-3 closely related to Staphylococcus sciuri by 98.0% - 99.0% similarity ; （2） the two strains could grow well at 0% -9.0% CaCl2 concentration; （3） the optimum temperatures of B723-1 and B723- 3 were 20 -35℃ and 25 -37℃ respectively and both could grow well at pH 7.0 - 10.0; （4） intracellular free amino acids could be rapidly accumulated in the strains under 1.8%, 3.6% , 5.4% and 9.0% CaCl2 stresses for 45rain, while intracellular protein in the strains under CaCl2 stress was higher than that in CaCl2-free strains and the membrane permeability of tolerant-CaCl2 strain under CaCl2 concentration stress lower than that of non-halophilic strain. Fig 4, Tab 1, Ref 11 .
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