中文摘要：

种植在不同生态区的烟草具有不同的香型。但香型物质形成在蛋白组水平的分子机制尚不清楚。本研究用基团标记技术（iTRAQ）对种植在两个不同生态区的烟草主要生育时期叶片中蛋白质的表达进行定量研究及生物信息学分析。结果表明：在鉴定到的2005个蛋白中,有291个在两个生态区烟草4个重要生长发育期叶片中的表达有显著差异,团棵期、旺长期、现蕾期和生理成熟期差异表达蛋白的数目分别为：64、65、90、72。这些差异表达蛋白质主要位于细胞基质、细胞组分以及细胞器中,主要参与代谢过程、细胞组成以及对外界刺激的应激过程;参与光合作用、次生代谢途径的蛋白质在两个生态区的生理成熟期烟叶中的表达差异显著。这暗示不同生态区的烟草叶片蛋白组表达模式不同,参与光合作用和次生代谢物质生物合成相关蛋白质的差异表达,导致不同生态区的烟叶中形成不同的香型物质。

英文摘要：

Tobacco plants grown in different ecological regions have different aromas. However, the proteomic mechanism of aromas formation is unknown. In this study, we used iTRAQ to identify differences in the constitutive proteomes of leaves collected from Nicotina tobacum L. plants grown in two typical ecological regions that produce distinct aroma of flue-cured tobaccos. We found that 291 out of a total of 2005 proteins expressions were significantly different between leaves harvested from the two regions in the four key developmental stages. Specifically, the expressions of 64 kinds of proteins in the rosette stage, 65 kinds of proteins in the rapid growh stage, 90 kinds of proteins in the budding stage, and 72 kinds of proteins in the physiologically maturing stage were different. The differentially expressed proteins mainly occur within the cell membrane, or as cellular components, or in organelles,and are involved in metabolic and cellular processes, and responds to external stimuli. Interestingly, the expression of some proteins involved in photosynthesis and secondary metabolite biosynthesis showed significant differences between leaves harvested from the two regions. These results suggest that the growh of tobacco in different ecological regions will result in different protein expression patterns of tobacco leaves, and that the differences in the expression of proteins involved in photosynthesis and secondary metabolite biosynthesis may largely underlie the differences in aroma.