中文摘要：

目的 进一步探讨miR-33对黑色素瘤B16F10细胞株增殖和凋亡的影响.方法 构建靶向miR-33的高表达mimics及inhibitor干扰单链,将同一株B16F10细胞分为5组：空白组、miR-33mimics组、mimics对照组、miR-33 inhibitor组、inhibitor对照组,运用脂质体转染技术将对应基因导入B16F10细胞中,从RNA水平和细胞水平分析miR-33对B16F10细胞增殖和凋亡的影响.结果 miR-33mimics组对应基因表达（1773.3±245.83）高于空白组,差异有统计学意义（P＜0.01）；miR-33 inhibitor组中对应基因相对表达（0.6973±0.1958）低于空白组和inhibitor对照组,但差异无统计学意义；miR-33mimics组与空白组相比细胞生长呈下降趋势,在转染后48 h（1.1875±0.0502）及72 h（1.7500±0.0933）趋势明显,差异均有统计学意义（均P＜ 0.05）；miR-33 mimics组细胞凋亡率[（1.8050±0.2050）％]高于空白组[（11300±0.1414）％]（P＜0.05）；与空白组相比,miR-33 mimics组G1期细胞比例[（62.7000±1.7321）％]增加,S期细胞比例[（23.4000±2.5044）％]减少,差异均有统计学意义（均P＜ 0.05）.结论 miR-33在高表达时抑制B16F10细胞增殖,促进细胞凋亡.

英文摘要：

Objective To further approach the effect of miR-33 to melanoma cells line B16F10 proliferation and apoptosis.Methods Constructing targeted miR-33 over-expression mimics and inhibitor,the same B16F10 cells were divided into five groups,control group,miR-33 mimics group,mimics control group,miR-33 inhibitor group,inhibitor control group,then gene transfer technology was used to transfer corresponding gene into B16F10 cells.The effect of miR-33 on B16F10 cell＇ s proliferation and apoptosis were analysed.Results The relative miR-33 gene expression of miR-33 mimics group （1773.3±245.83） was higher than that of control group,which had statistical significance （P 〈 0.01）.The gene expression of miR-33 inhibitor group （0.6973±0.1958） was lower than those of control group and inhibitor control group.The cell growth rate of miR-33 mimics group was lower than those of control group and the trend after transfection 48 h （1.1875±0.0502） and 72 h （1.7500±0.0933） was significant （P 〈 0.05）.The cell apoptotic ratio of miR-33 mimics group [（1.8050±0.2050） %] was higher than that of control group [（1.13000±0.1414） %] （P 〈 0.05）.Compared with control group the cell proportion ofG1 period in miR-33 mimics group [（62.7000±1.7321） %]increased,the cell proportion of S period [（23.4000±2.5044） %] decreased,both of them had statistical significance （both P 〈 0.05）.Conclusion miR-33 over-expression can restrain the proliferation of B16F10 cells line,promote B16F10 cells＇ apoptosis.