中文摘要：

目的探讨维生素C（VC）对甲醛诱导胎鼠发育毒性的影响。方法应用体外全胚胎培养模型,观察100μg/mL VC对终浓度为9,27,81μmol/L甲醛诱导昆明种小鼠胚胎发育毒性的影响。结果与对照组比较,9μmol/L甲醛可使胚胎头长、颅臀长、谷胱甘肽（GSH）、丙二醛（MDA）含量分别升高到（1.66±0.21）,（3.48±0.40）mm,（47.70±2.12）mg/（g.prot）,（4.27±0.62）nmol/（mg.prot）,差异有统计学意义（P〈0.05） 27μmol/L甲醛使胚胎脏层卵黄囊（VYS）直径、头长、颅臀长、体节数、GSH含量分别下降到（4.35±0.65）,（1.48±0.30）,（3.19±0.43）mm,（25.2±1.2）对,（40.25±4.38）mg/（g.prot）,MDA含量升高到（6.29±0.47）nmol/（mg.prot）,与对照组比较,差异有统计学意义（P〈0.05） VC能明显改善上述指标,但与对照组比较差异无统计学意义（P〈0.01） VC不能明显改善81μmol/L甲醛诱导的毒性损伤。结论VC对一定剂量范围内的甲醛胚胎毒性具有明显抑制作用。

英文摘要：

Objective To observe the inhibitive effect of vitamin C（VC） on embryonic development toxicity induced by formaldehyde in mice. Methods Kunming mice embryos were incubated together with formaldehyde （ 9, 27, and 81 μmol/L） and VC（ 100 μg/mL） ,respectively, with whole embryo culture method. The development of mice embryos was then assessed. Results When the concentration of formaldehyde was 9μmol/L, the increases of head lengh （ 1.66 ± 0.21 mm）, cranial arm length （ 3.48 ± 0. 40mm ）, glutathione （GSH） （ 47.70 ± 2. 12mg/g prot ） and malonaldehyde （MDA） （4. 27 ±0. 62nmol/mg · prot） were significantly different（P 〈0. 05） from those of the control group. When the concentration was 27 μmol/L, the increases of embryo visceral yolk sac （VYS） diameter（ 4. 35 ± 0. 65mm）, head lengh （ 1. 48 ±0. 30 mm）, cranial arm length （ 3. 19 ± 0. 43mm ）, number of arthromera pairs （ 25. 2 ± 1.2 ） and GSH （40. 25 ±4. 38mg/g · prot） and the decrease of MDA（6. 29 ±0. 47nmol/mg· prot） were observed with significant differences （ P 〈 0. 05 ） compared with that of the control group. VC could improve the indexes but there was no significant difference between the control group and the VC group （P 〉 0. 05 ）. The toxic injury induced by formaldehyde （81 μmol/L） could not be improved with VC significantly. Conclusion VC inhibits embryonic development toxicity induced by certain dose of formaldehyde.